J Dent Hyg Sci.  2023 Dec;23(4):389-395. 10.17135/jdhs.2023.23.4.389.

Antibacterial Efficacy of Dental Sealant Containing Phytoncide

• Yang SY
• Lee MJ

• KMID: 2550551
• DOI: http://doi.org/10.17135/jdhs.2023.23.4.389

Abstract

Background
Dental caries prevention is a key research focus in dentistry, requiring advancements in the formulation of dental sealants. This study investigated the physical and antibacterial attributes of dental sealant enriched with phytoncide.
Methods
Phytoncide was mixed with a commercially available dental sealant (Clinpro) at concentrations of 0 (control), 1.5%, 3%, and 4.5% by weight (wt%). The flexural strength, curing depth, and wettability of the dental sealant were measured. Antibacterial properties against Streptococcus mutans were evaluated through the enumeration of colony-forming units. Statistical analyses employed one-way variance analysis followed by Tukey’s test (p＜0.05).
Results
The dental sealant containing 3% phytoncide showed no significant difference in flexural strength and curing depth compared with that in the control group (p＞0.05). The flexural strength and curing depth decreased with increasing phytoncide content and significantly differed in sealant containing 4.5 wt% phytoncide (p＜0.05). Wettability did not differ between the experimental and control groups (p＞0.05). The antibacterial properties of the sealant containing 1.5% phytoncide were the same as those of the control group (p＞0.05). The bacterial viability was significantly reduced in groups containing 3% and 4.5% phytoncide compared with that in the control group (p＜0.05).
Conclusion
Dental sealants incorporating phytoncide have a promising potential as antibacterial dental materials.

Keyword

Antibacterial efficacy; Dental sealant; Phytoncide; Streptococcus mutans