J Mycol Infect.
2023 Jun;28(2):42-53. 10.17966/JMI.2023.28.2.42.
Red Ginseng Water Extract Aggravates Inflammation in Sebocytes and Outer Root Sheath Cells after Treatment with Lipopolysaccharide and Mice with Cutibacterium acnes-induced Inflammatory Nodules
- Affiliations
-
- 1Department of Immunology, School of Medicine, Kyungpook National University, Daegu, Korea
- 2BK21 FOUR KNU Convergence Educational Program of Biomedical Sciences for Creative Future Talents, School of Medicine, Kyungpook National University, Daegu, Korea
- 3Department of Dermatology, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu, Korea
- KMID: 2549621
- DOI: http://doi.org/10.17966/JMI.2023.28.2.42
Abstract
- Background
Ginseng, a well-known health-supportive herbal medicine in Korea, has various forms, including red ginseng (RG) produced through steaming and drying white ginseng. RG has been associated with numerous protective functions in various diseases.
Objective
The objective of this study was to investigate whether RG water extract exacerbates inflammation in human sebocytes and outer root sheath (ORS) cells following treatment with lipopolysaccharide (LPS) and Cutibacterium acnes strain (ATCC 1182)-induced inflammatory nodules in mice.
Methods
Sebocytes and ORS cells were isolated and cultured from the human scalp. Reverse transcriptionpolymerase chain reaction and enzyme-linked immunosorbent assay were used to measure the expression of inflammatory cytokine and sebum-related genes after treatment with RG and LPS. Mice with Cutibacterium acnesinduced inflammatory nodules were orally administered RG in water for two weeks, and immunofluorescence staining was performed to assess the inflammatory nodules.
Results
RG enhanced LPS-mediated inflammation by increasing the mRNA and protein expression of interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor-α in sebocytes and ORS cells. RG also upregulated the expression of p-nuclear factor-kappa B (p-NFκB), p-c-Jun, and c-Jun N-terminal kinase (p-JNK) in LPS-treated sebocytes and ORS cells. Moreover, RG promoted LPS-induced sebum production in sebocytes. Additionally, RG hindered the improvement of inflammatory nodules in mice and increased the expression of inflammatory biomarkers, such as neutrophil, myeloperoxidase, and IL-1β, as well as tissue remodeling biomarkers, such as myeloperoxidase (MMP)-2, MMP-3, and MMP-9 in the inflammatory nodules.
Conclusion
Our findings strongly suggest that RG exacerbates acne vulgaris. It is advisable to avoid using RG in patients with inflammatory acne.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Red Ginseng Acidic Polysaccharides Promote the Expression of Acne-Related Inflammatory Biomarkers in Lipopolysaccharide-Treated Sebocytes and Outer Root Sheath Cells and Cutibacterium acnes-Injected Mice
- Dieckol Inhibits the Effects of Particulate Matter 10 on Sebocytes, Outer Root Sheath Cells, and Cutibacterium Acnes−Pretreated Mice
- Effect of Red Ginseng Oil on Cultured Sebocytes and Outer Root Sheath Cells after Treatment with Lipopolysaccharide
- Effects of Black Ginseng Water Extract under the Inflammatory Conditions of Cultured Sebocytes and Outer Root Sheath Cells
- Effects of <10-μm Particulate Matter on Cultured Human Sebocytes and Outer Root Sheath Cells and Usefulness of Siegesbeckia Herba Extract
