Korean J Otorhinolaryngol-Head Neck Surg.  2023 Dec;66(12):829-835. 10.3342/kjorl-hns.2023.00577.

Effect of Lipopolysaccharide on Development of Aspergillus fumigatus Biofilm on Nasal Epithelial Cells

• Yoo JH
• Kim TG
• WhangBo CH
• Ye MK
• Shin SH

• KMID: 2549464
• DOI: http://doi.org/10.3342/kjorl-hns.2023.00577

Abstract

Background and Objectives
Aspergillus fumigatus is one of the common causes of fungal airway inflammatory diseases and lipopolysaccharide (LPS) acts as key regulator of airway inflammation. In addition, bacterial and fungal biofilm commonly coexist in chronic rhinosinusitis. In this study, we evaluated the effect of LPS on the development of A. fumigatus biofilm formation on the nasal epithelial cells. Materials and Method Primary nasal epithelial cells were cultured with A. fumigatus conidia with or without LPS for 5 days. The production of interleukin (IL)-6, IL-8, and transforming growth factor (TGF)-β1 from nasal epithelial cells was determined by enzyme-linked immunosorbent assay. The effects of LPS on A. fumigatus biofilm formation were determined using biofilm dry weight, and crystal violet, concanavalin A, safranin staining, and confocal scanning laser microscopy.
Results
LPS and A. fumigatus significantly enhanced the production of I L-6, I L-8, and TGF-β1 from nasal epithelial cells. can form biofilm on primary nasal epithelial cells, and this significantly increased in a time-dependent manner when cocultured with LPS, the dry weight, concanavalin A, and safranin staining.
Conclusion
The exposure of A. fumigatus to LPS enhanced the formation of biofilms. The coexistence of LPS and A. fumigatus enhanced fungal biofilm formation and this may be associated with the development of recalcitrant airway inflammatory diseases.

Keyword

Biofilm; Epithelial cells; Lipopolysaccharide