Restor Dent Endod.  2022 Aug;47(3):e31. 10.5395/rde.2022.47.e31.

Cytotoxicity of two self-adhesive resin cements and their interference in the phagocytic activity of murine macrophages

• Silva DCd
• Vaz LG
• Tavares WLF
• Vieira LQ
• Oliveira RRd
• Sobrinho APR

• KMID: 2548135
• DOI: http://doi.org/10.5395/rde.2022.47.e31

Abstract

Objectives
This study aimed to evaluate in vitro the effects of the self-adhesive resin cements RelyX U200 (3M ESPE) and seT PP (SDI Limited) on murine macrophages and the interference of the photoactivation.
Materials and Methods
Cell viability assays, cell adherence, yeast phagocytosis of Saccharomyces boulardii and production of reactive oxygen species (ROS) were performed in the presence of capillaries containing the respective self-adhesive cement when photoactivated or not.
Results
After long periods of contact, both types of cements, when not photoactivated, are more cytotoxic for macrophages. The seT PP cement when only chemically activated seems to interfere more negatively in the process of phagocytosis of yeasts S. boulardii. Both types of cements interfere in the cell adhesion process, independent of photoactivation. None of the types of cements tested was able to induce the production of ROS.
Conclusions
Our results highlight the great importance of the photoactivation of selfadhesive resin cements in the dental clinic, since RelyX U200, when photoactivated, presented the best results within the evaluated parameters.

Keyword

Cytotoxicity; Macrophages; Dental cement; Resin cement

Figure

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  Figure 1 Percentage of living macrophages from wt C57BL/6 mice after incubation in polypropylene tubes with capillaries containing self-adhesive resin cement from commercial sources. Bars represent the mean of three experiments in triplicate; lines stand for the standard error of the means.*The statistical difference p < 0.05.

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  Figure 2 Percentage of adherent macrophages from wt C57BL/6 mice after incubation in culture plates with capillaries containing self-adhesive resin cement from both commercial sources. Bars stand for the mean results of two experiments made in triplicate; lines indicate standard error of the means.*The statistical difference between control group and all experimental groups.

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  Figure 3 Numbers of yeast cells per macrophages are also shown. Bars stand for the mean results of two experiments made in Triplicates; lines indicate standard error of the means.*The statistical difference p < 0.05.

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  Figure 4 Kinetic of reactive oxygen intermediates production by macrophages from wt C57BL/6 mice (A). Reactive oxygen species mean production by macrophages (B). Cells were cultured with capillaries containing self-adhesive resin cement and stimulated with zymosan. Bars stand for the mean results of two experiments made in triplicate; lines indicate standard error of the means.*The statistical difference between control group and all experimental groups p < 0.05.

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