J Vet Sci.
2023 Sep;24(5):e55. 10.4142/jvs.22288.
Inhibition of caspase-1-dependent apoptosis suppresses peste des petits ruminants virus replication
- Affiliations
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- 1College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China
- 2College of Animal Science and Veterinary Science, Qinghai University; Qinghai Key Laboratory of Pathogen Diagnosis and Green Prevention and Control Technology for Animal Diseases, Xining 810016, China
- 3State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
- KMID: 2546799
- DOI: http://doi.org/10.4142/jvs.22288
Abstract
- Background
Peste des petits ruminants (PPR), caused by the PPR virus (PPRV), is an acute and fatal contagious disease that mainly infects goats, sheep, and other artiodactyls. Peripheral blood mononuclear cells (PBMCs) are considered the primary innate immune cells.
Objectives
PBMCs derived from goats were infected with PPRV and analyzed to detect the relationship between PPRV replication and apoptosis or the inflammatory response.
Methods
Quantitative real-time polymerase chain reaction was used to identify PPRV replication and cytokines expression. Flow cytometry was conducted to detect apoptosis and the differentiation of CD4+ and CD8+T cells after PPRV infection.
Results
PPRV stimulated the differentiation of CD4+ and CD8+ T cells. In addition, PPRV induced apoptosis in goat PBMCs. Furthermore, apoptosis and the inflammatory response induced by PPRV could be suppressed by Z-VAD-FMK and Z-YVAD-FMK, respectively. Moreover, the virus titer of PPRV was attenuated by inhibiting caspase-1-dependent apoptosis and inflammation.
Conclusions
This study showed that apoptosis and the inflammatory response play an essential role in PPR viral replication in vitro, providing a new mechanism related to the cell host response.
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