Korean J healthc assoc Infect Control Prev.  2023 Jun;28(1):178-179. 10.14192/kjicp.2023.28.1.178.

PFGE, Rep-PCR, & NGS: The Most Beautiful and Oldest Thing is Only in Dreams

• Park KU

• KMID: 2543149
• DOI: http://doi.org/10.14192/kjicp.2023.28.1.178

Figure

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  Fig. 1 Pulsed-field gel electrophoresis showed that the bacteria isolated from the two patients were of the same strain (A-C). The DNA bands of different strains of Serratia marcescens (D-L) showed different band patterns compared with A, B, and C. (A) Organism cultured and isolated from vitreous biopsy during vitrectomy of case 1. (B) Organism cultured and isolated from vitreous tap of case 2. (C) Organism cultured and isolated from vitreous biopsy during vitrectomy of case 2. (D-L) S. marcescens of other strains. *Pseudomonas aeruginosa ATCC27853; M Lambda ladders.

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  Fig. 2 Comparison of the single-nucleotide polymorphism-based strain typing results of short-read next-generation sequencing (SR-NGS) and long-read next-generation sequencing (LR-NGS) for vancomycin-resistant Enterococcus faecium. (A, B) Depict the maximum-likelihood phylogenies constructed using SR- and LR-NGS, respectively. The heatmaps on the right show the van gene clusters. (C) Shows a tanglegram of the two phylogenetic trees.

Reference

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2. Koh WJ, Jeong BH, Jeon K, Kim SY, Park KU, Park HY, et al. 2016; Oral macrolide therapy following short-term combination antibiotic treatment of Mycobacterium massiliense lung disease. Chest. 150:1211–21. DOI: 10.1016/j.chest.2016.05.003. PMID: 27167209.

3. Oh S, Nam SK, Chang HE, Park KU. 2022; Comparative analysis of short- and long-read sequencing of vancomycin-resistant enterococci for application to molecular epidemiology. Front Cell Infect Microbiol. 12:857801. DOI: 10.3389/fcimb.2022.857801. PMID: 35463637. PMCID: PMC9019564.