Lab Med Online.  2022 Jan;12(1):74-78. 10.47429/lmo.2022.12.1.74.

A Case of Bacteremia Caused by Pantoea ananatis in a Patient Receiving Home Parenteral Nutrition

• Lee J
• Park JH
• Kim TS
• Park H

• KMID: 2538588
• DOI: http://doi.org/10.47429/lmo.2022.12.1.74

Abstract

Pantoea ananatis is a Gram-negative, rod-shaped, non-spore-forming, motile bacterium that belongs to the family Erwiniaceae of the order Enterobacterales. In this study, we report the first Korean case of bacteremia caused by P. ananatis isolated from a 17-year-old female hospitalized due to fever. She had undergone total colectomy and massive intestinal resection due to intestinal pseudo-obstruction. Subsequently, the patient received home parenteral nutrition with a central venous catheter (CVC) for ten years and was hospitalized several times for central line-associated bloodstream infections. Gram-negative rods were isolated from two pairs of CVC blood cultures collected at admission. The isolate was identified as Pantoea spp. (95.0%) by GN ID card of the VITEK 2 system (bioMérieux, USA). It was identified as P. ananatis (score: 2.328) by matrix-assisted laser desorption ionization time-of-flight mass spectrometry using MALDI Biotyper (Bruker Daltonics, Germany). We used universal primers to perform 16S rRNA gene sequence analysis. The sequence was searched in the GenBank database and showed 99.32% identity with P. allii and 99.18% identity with P. ananatis. Since the result was not species-specific, additional cpn60 gene sequencing was performed. The sequence revealed 99.91% identity with P. ananatis and 94.00% identity with P. allii. Consequently, the isolate was confirmed as P. ananatis. To accurately identify Pantoea spp., cpn60 was more reliable than the 16S rRNA gene. This is the first confirmed case of bacteremia caused by P. ananatis in Korea.

Keyword

Pantoea ananatis; Bacteremia; MALDI-TOF MS; 16S rRNA; cpn60

Figure

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  Fig. 1 Microscopic image and colony morphology of Pantoea ananatis. (A) Gram-negative non-spore-forming rods (Gram stain, ×1,000). (B) Round, convex, and pale-yellow colonies on a blood agar plate.

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