J Biomed Transl Res.  2022 Dec;23(4):215-234. 10.12729/jbtr.2022.23.4.215.

Effects of PB203 on the skin photoaging of ultraviolet B (UVB)-irradiated hairless mice and human keratinocytes

Affiliations
  • 1R&D Center, G&P Bioscience Co., Ltd., Seoul 07528, Korea
  • 2Department of Dermatology, College of Medicine, Chung-Ang University, Seoul 06974, Korea
  • 3Department of Medicine, Graduate School, Chung-Ang University, Seoul 06974, Korea

Abstract

Repetitive or excessive exposure to ultraviolet (UV) radiation causes oxidative stress-mediated skin photoaging through the overproduction of reactive oxygen species. Actinidia polygama is known as a medical plant used in oriental medicine for treating several diseases such as abdominal pain, stroke and rheumatoid arthritis. Recently, it was reported that A. polygama extract had anti-wrinkle and skin hydrating properties in ultraviolet B (UVB)-exposed hairless mice. However, the molecular biological mechanism of this extract on alleviating skin photoa-ging is still unknown. Therefore, we investigated the anti-photoaging effects of PB203, which is the powder of A. polygama extract, in the in vivo and in vitro photoaging models. First, PB203 showed 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activities due to the presence of anti-oxidant components including flavo-noids and polyphenols. In UVB-irradiated hairless mice, oral administration of PB203 (100 mg/ kg) significantly improved wrinkle formation, skin dehydration, elasticity and skin barrier func-tion by decreasing the levels of matrix metalloproteinases (MMPs) and increasing those of col-lagen I, filaggrin, involucrin and loricrin. Especially, the reduced production of p-p38, p-c-Jun and p-c-Fos by PB203 reversed the elevated levels of MMPs mediated by UVB exposure, re-sulting in the upregulation of collagen I expression. Consistent with these animal data, PB203 remarkably enhanced the mRNA expression of collagen I, filaggrin, involucrin and loricrin,while suppressed that of MMPs in UVB-irradiated HaCaT cells. And PB203 increased the wound recovery rate of cells by promoting their proliferation and migration. Moreover, PB203 significantly recovered the activity of superoxide dismutase inhibited by UVB in both mice and cells. In conclusion, PB203, which protects skin from UVB-induced photodamage by exerting antioxidant properties, can be considered to have sufficient potential as a functional ingredient or therapeutic agent improving skin photoaging and related skin symptoms.

Keyword

A. polygama extract (PB203); photoaging; matrix metalloproteinases (MMPs); collagen I; filaggrin proteins
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