J Vet Sci.  2022 Nov;23(6):e82. 10.4142/jvs.22178.

Comparative in vitro biotransformation of fipronil in domestic poultry using liver microsome

• Khidkhan K
• Poapolathep S
• Kulprasertsri S
• Sukkheewan R
• Khunlert P
• Giorgi M
• Poapolathep A

• KMID: 2536704
• DOI: http://doi.org/10.4142/jvs.22178

Abstract

Domestic poultry are among the non-target species of exposure to fipronil, but limited information is available on the metabolic effects of fipronil exposure in avian. We investigated the comparative capacity of in vitro biotransformation of fipronil among chicken, duck, quail, goose, and rat. Interspecies differences in kinetic parameters were observed; the clearance rate calculations (Vmax/Km) indicated that chicken and duck are more efficient in the cytochrome P450-mediated metabolism of fipronil to sulfone than quail, goose and rat. The lower hepatic clearance of fipronil in quail, goose and rat, suggested that fipronil sulfone may serve as a biomarker to indicate fipronil exposure in these species.

Keyword

Biotransformation; cytochrome P450; sulfone; microsome; poultry