Korean J Transplant.  2022 Nov;36(Supple 1):S44. 10.4285/ATW2022.F-1581.

Fat and protein modification affects the repair process in ischemic acute kidney injury

Affiliations
  • 1Department of Nephrology, Samsung Medical Center, Seoul, Korea
  • 2Department of Pathology, Samsung Medical Center, Seoul, Korea

Abstract

Background
The effects of dietary fat and protein on the repair process after ischemic acute kidney injury (AKI) are not well established. In this study, we investigated the effects of dietary fat and protein modification on intrarenal immunologic micromilieu and the repair of postischemic kidneys using murine ischemic AKI and human kidney-2 (HK-2) cell hypoxia model.
Methods
Control diet, high-fat with high-protein (HF+HP) diet, and low-fat with low protein (LF+LP) diet were provided to 9-weekold male C57BL/6 mice from day 3 after bilateral or unilateral ischemia-reperfusion injury (BIRI or UIRI) operation. Hypoxic HK-2 cells were treated with additional lipid or amino acid.
Results
Body weight and total cholesterol concentration were higher in the HF+HP group than in the control group. Body weight, blood pressure, and BUN were lower in the LF+LP group compared to the control group, while there were no differences in plas-ma creatinine between groups. In the expression of intrarenal cytokines/chemokines after BIRI, RANTES was increased in the HF+HP group, and IFN-γ, IL-4, IL-10, IL-6, and TNF-α were increased and VEGF was decreased in the LF+LP group. IFN- γ, IL4, and IL-6 were decreased in the HF+HP group and IL-6 was increased in the LF+LP group compared to the control group after UIRI. Postischemic kidneys of the LF+LP group showed more extensive fibrosis compared to the control group after UIRI Proliferation of HK-2 cells after hypoxic insult was suppressed by additional amino acid, but facilitated by additional lipid.
Conclusions
This study demonstrated that extreme restriction of protein and fat during the recovery phase of ischemic AKI can be detrimental. Further studies are required to elucidate the optimal dietary compositions and the individual effects of protein and lipid during the recovery of ischemic AKI.

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