Tissue Eng Regen Med.  2022 Aug;19(4):703-715. 10.1007/s13770-022-00460-6.

Preparation and Biocompatibility Study of Contrast-Enhanced Hernia Mesh Material

• Ding X
• Zhu J
• Liu A
• Guo Q
• Cao Q
• Xu Y
• Hua Y
• Yang Y
• Li P

• KMID: 2533909
• DOI: http://doi.org/10.1007/s13770-022-00460-6

Abstract

BACKGROUND
Meshes play a crucial role in hernia repair. However, the displacement of mesh inevitably leads to various associated complications. This process is difficult to be traced by conventional imaging means. The purpose of this study is to create a contrast-enhanced material with high-density property that can be detected by computed tomography (CT).
METHODS
The contrast-enhanced monofilament was manufactured from barium sulfate nanoparticles and medical polypropylene (PP/Ba). To characterize the composite, stress tensile tests and scanning electron microscopy (SEM) was performed. Toxicity and biocompatibility of PP/Ba materials was verified by in vitro cellular assays. Meanwhile, the inflammatory response was tested by protein adsorption assay. In addition, an animal model was established to demonstrate the long-term radiographic effect of the composite material in vivo. Subsequent pathological tests confirmed its in vivo compatibility.
RESULTS
The SEM revealed that the main component of the monofilament is carbon. In vitro cell experiments demonstrated that novel material does not affect cell activity and proliferation. Protein adsorption assays indicated that the contrast-enhanced material does not cause additional inflammatory responses. In addition, in vivo experiments illustrated that PP/Ba mesh can be detected by CT and has good in vivo compatibility.
CONCLUSION
These results highlight the excellent biocompatibility of the contrast-enhanced material, which is suitable for human abdominal wall tissue engineering.

Keyword

Contrast-enhanced mesh; Hernia repair; Nano barium sulfate; Polypropylene