Med Lasers.  2020 Jun;9(1):51-57. 10.25289/ML.2020.9.1.51.

Design and Evaluation of a Scalding Animal Model by the Boiling Water Method

• Hua C
• Lyu L
• Ryu HS
• Park SY
• Lim NK
• Abueva C
• Chung PS

• KMID: 2533465
• DOI: http://doi.org/10.25289/ML.2020.9.1.51

Abstract

Background and Objectives
For experiments on simulated burn, the preparation of an animal model is a very important step. The purpose of the current experiment is to design a simple and controllable method for the preparation of thirddegree scald in a mouse model using the boiling water method.
Materials and Methods
A total of 18 Swiss mice were used. After the anesthetization, the mice were scalded by boiling water (100°C) using a mold with a 1 cm 2 circle area on the dorsum at contact times of 3s, 5s, and 8s. After confirming that 8 seconds of scald can cause a third-degree scald, the skin samples were collected at day 2, 4, 6, 8, 10, and 12, and analyzed by histopathological examinations. The wound retraction index (WRI) was also measured.
Results
Third-degree scald involving full-thickness skin was observed in the 8-second scald group, while a 3-second scald caused a superficial second-degree scald and a 5-second scald caused a deep seconddegree scald. After third-degree scald, the burn wound continued to contract until day 14.
Conclusion
The scalding model of mice can be successfully established by the boiling water method. This method is easy to operate, it has a low cost, and it can control the scald depth by controlling the scald time. This is adequate to study skin thermal injury in the future. The scald model established by this method can last for 14 days.

Keyword

Mouse model; Burn; Scald