J Vet Sci.  2022 Sep;23(5):e72. 10.4142/jvs.22064.

Hydrogen sulfide protects from acute kidney injury via attenuating inflammation activated by necroptosis in dogs

Affiliations
  • 1College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, P. R. China
  • 2Heilingjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Northeast Agricultural University, Harbin 150030, P. R. China

Abstract

Background
The treatment of acute kidney injury (AKI), a common disease in dogs, is limited. Therefore, an effective method to prevent AKI in veterinary clinics is particularly crucial.
Objectives
Hydrogen sulfide (H 2 S) is the third gaseous signal molecule involved in various physiological functions of the body. The present study investigated the effect of H 2 S on cisplatin-induced AKI and the involved mechanisms in dogs.
Methods
Cisplatin-injected dogs developed AKI symptoms as indicated by renal dysfunction and pathological changes. In the H 2 S-treated group, 50 mM sodium hydrosulfide (NaHS) solution was injected at 1 mg/kg/h for 30 min before cisplatin injection. After 72 h, tissue and blood samples were collected immediately. We performed biochemical tests, optical microscopy studies, analysis with test kits, quantitative reverse-transcription polymerase chain reaction, and western blot analysis.
Results
The study results demonstrated that cisplatin injection increased necroptosis and regulated the corresponding protein expression of receptor interacting protein kinase (RIPK) 1, RIPK3, and poly ADP-ribose polymerase 1; furthermore, it activated the expressions of inflammatory factors, including tumor necrosis factor-alpha, nuclear factor kappa B, and interleukin-1β, in canine kidney tissues. Moreover, cisplatin triggered oxidative stress and affected energy metabolism. Conversely, an injection of NaHS solution considerably reduced the aforementioned changes.
Conclusions
In conclusion, H 2 S protects the kidney from cisplatin-induced AKI through the mitigation of necroptosis and inflammation. These findings provide new and valuable clues for the treatment of canine AKI and are of great significance for AKI prevention in veterinary clinics.

Keyword

Acute kidney injury; hydrogen sulfide; necroptosis; inflammation; dogs
Full Text Links
  • JVS
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr