J Dent Hyg Sci.  2022 Sep;22(3):164-170. 10.17135/jdhs.2022.22.3.164.

Autophagy May Mediate Cellular Senescence by Nicotine Stimulation in Gingival Fibroblasts

• Jun NR
• Jang JH
• Lee JY
• Lee SI

• KMID: 2533373
• DOI: http://doi.org/10.17135/jdhs.2022.22.3.164

Abstract

Background
When cells are damaged by nicotine, cellular senescence due to oxidative stress accelerates. In addition, stress-induced inflammatory response and cellular senescence cause the accumulation of damaged organelles in cells, and autophagy appears to remove them. Conversely, when autophagy is reduced, harmful cell components accumulate, and aging is accelerated. This study aimed to determine the association between nicotine-induced cellular senescence and autophagy expression patterns in human gingival fibroblasts.
Methods
Cells were treated with various concentrations of nicotine (0, 0.1, 0.5, 1, 2, and 5 mM) and 10 nM rapamycin was added to 1 mM nicotine to investigate the relationship between autophagy and cellular senescence. Cell viability was confirmed using WST-8 and the degree of cellular senescence was measured by SA-β-gal staining. The expression of the inflammatory proteins (COX-2 and iNOS) and autophagy markers (LC3-II, p62, and Beclin-1) was analyzed by western blotting.
Results
The cell viability tended to decrease in a concentration-dependent manner. COX-2 showed no concentration-dependent expression and iNOS increased in the 0.5 mM nicotine treated group. The degree of cellular senescence was the highest in the 1 mM nicotine treatment group. In the group treated with rapamycin and nicotine, the conversion ratio of LC3-II to LC3-I was the highest, that of p62 was the lowest, and the level of Beclin-1 proteins was significantly increased. Furthermore, the degree of cellular senescence was reduced in the group in which rapamycin was added to nicotine compared to that in the group treated with nicotine alone.
Conclusion
This study provides evidence that autophagy activated in an aging environment reduces cellular senescence to a certain some extent.

Keyword

Autophagy; Cellular senescence; Gingival fibroblasts; Nicotine; Periodontal disease