Yonsei Med J.
2022 Jul;63(7):648-656. 10.3349/ymj.2022.63.7.648.
Ovarian Tissue-Based Hormone Replacement Therapy Recovers Menopause-Related Signs in Mice
- Affiliations
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- 1Department of Biomedical Laboratory Science, Eulji University, Seongnam, Korea
- 2Department of Senior Healthcare, BK21 Plus Program, Graduate School of Eulji University, Seongnam, Korea
- 3Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea
- 4Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea
- 5Program in Nanoscience and Technology, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, Korea
- 6Department of Applied Bioengineering, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, Korea
- 7Eulji Medi-Bio Research Institute (EMBRI), Eulji University, Daejeon, Korea
- KMID: 2530689
- DOI: http://doi.org/10.3349/ymj.2022.63.7.648
Abstract
- Purpose
In women, menopause manifests with a variety of symptoms related to sex-hormone deficiency. Supplementing steroid hormones with pharmacological drugs has been widely practiced. However, considering the possible complications associated with artificial hormone therapy, studies have been conducted to find an alternative to pharmacological hormone replacement therapy. Accordingly, this study aimed to evaluate the efficacy of tissue-based hormone replacement therapy (tHRT) for treating post-menopausal signs and symptoms.
Materials and Methods
CD-1 mice were ovariectomized, and the ovaries were cryopreserved. Following artificial induction of post-menopausal osteoporosis, cryopreserved ovaries were subcutaneously autografted, and indexes related to bone health were monitored for 12 weeks. Bone mineral density (BMD), bone mineral contents (BMC), total bone volume (BV), and body fat mass were measured by dual energy X-ray absorptiometry. Uterine atrophy was assessed histologically, and bone microstructures were imaged by micro-computed tomography analysis.
Results
Regardless of the number of grafted ovaries, the BMC, BMD, and BV values of mice that underwent ovary transplantation were better than those that did not undergo transplantation. The uteruses in these mice were thicker and heavier after auto-transplantation. Furthermore, the bone microstructure recovered after tHRT.
Conclusion
Recovery of menopause-related bone loss and uterine atrophy was achieved through tHRT. Ovarian tissue cryopreservation and transplantation may be applicable not only in patients wanting to preserve fertility but also in sex hormone-deficient post-menopausal women.
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