Tuberc Respir Dis.  1972 Jun;19(2):20-29. 10.4046/trd.1972.19.2.20.

An Experimental Study of the Effect of Simultaneous Vaccination with B. C. G. and Smallpox Vaccine -Part 2. Experiments in the innunized aminals and the rabbit kidney fibroblast monolayer cell culture

• KMID: 2529074
• DOI: http://doi.org/10.4046/trd.1972.19.2.20

Abstract

The objective of BCG and smallpox vaccination is to induce acquired resistance by artifícial infection with a harmless from of the tubercle bacillus and vaccinia virus. If applied properly on a large scale, it is the easiest, most economical, most time-saving, and most effective measure to reduce the tuberculosis problem (remembering that there has been no reported case of smallpox since 1958 in this country). However, in this country with a high prevalence of tuberculosis, (3) the effectiveness of BCG vaccination in reducing the incidence of tuberculosis in a population which is susceptible is related to the protective value or potency of the BCG vaccine and to skilled vaccination. The purpose of in this experimental study is based on following hypothesis: Even with BCG vaccination alone some untoward side reactions may be observed. Therefore, more side-effects would be expected at the vaccination region as well as systemically if the smallpox vaccination is given simultaneously with the BCG vaccination. BCG and Vaccinia virus are all live microbes: therefore, if they interfere with each other inside the human body, impedíments to the immunizing process may occur with the pathological view the authors of this study used guinea-pigs, rabbits, and mice: also they tested some of the monolayer cell culture from the BCG and Vaccinia virus immunized rabbit kidney fibroblast, in order to examine the effect of smallpox and BCG vaccination on the acquisition of immunity against smallpox and tuberculosis. The result are summarized as follows: 1. The result of pock counts of vaccinia, no normal trend of quantitative culture. Reisolated the vaccinia virus inoculated in the BCG immunized group of rabbits, guinea-pigs and mice. 2. Result of viability count of BCG was irreconcilable trend in quantitative culture. Reisolated the BCG inoculated in the vaccinia immunized group of the rabbits, guinea-pigs and mice. 3. At the necropsy of the BCG and vaccinia virus interchanged inoculation group of BCG and vaccinia virus immunized rabbits, guinea-pigs and mice, no significant difference between the groups was observed in the degree of tuberculous or vaccinal pathogenic signs. However, in most of immunized test groups were observed pathogenic changes in organs: blood-shot, fatty degeneration, lymph gland saturated, and macrophage increased. in the lung, liver, spleen, kidney and lymph glands, when compared to the control groups which were within normal limits. 4. Viability count of BCG and vaccinia pocks, decreased number of quantitative culture in both of the test groups. Reisolated the monolayer cell culture of rabbit kidney fibroblast with the immunized rabbit serum tested groups. 5. The test group with immunized rabbit serum showed a decrease in reisolated BCG and vaccinia compared to the test group without immunized rabbit serum. The present investigation seems to suggest the possibility of such from the increase in the local reaction and decrease in the acquisition of immunity, caused by either immune anti-body in the vaccinated human.