Tuberc Respir Dis.  1980 Jun;27(2):45-52. 10.4046/trd.1980.27.2.45.

Growth of Various Mycobacteria in Dubos Liquid Medium, Hyaluronic Acid Supplemented Medium and Cholesterol Supplemented Medium

• KMID: 2528882
• DOI: http://doi.org/10.4046/trd.1980.27.2.45

Abstract

In order to compare the growth of various cultivable Mycobacteria in cholesterol or hyaluronic acid supplemented media with that in ordinary Dubos liquid medium , some species of Mycobacteria, including human pathogens and non-pathogens (M. tuberculosis, B.C.G., M. vaccae, M. kansasii, M. scrofulaceum) were cultivated in three different media: Dubos liquid medium, KI-1 medium containing 100 μg/ml cholesterol and 10% sheep serum (Kato & Ishaque, 1978), and modified LA-3 medium containing 0.1% hyaluronic acid and 1% bovine serum albumin (Skinsnes, 1974). The cultivation of Mycobacteria in the three different media were compared by measurement of dry weight of bacterial growth. The effects of bovine serum albumin on cholesterol utilization by Mycobacteria were also examined by modifying the constituents of KI-1 medium. The results are summarized as follows; 1. All of above Mycobacteria examined in this study grew better in KI-1 medium than in others. This was especially true when bacterial growth in KI-1 medium was compared with that in Dubos medium: 4 and 3 fold increases in M. tuberculosis were observed in KI-1 medium within 4 to 6 weeks, 10 and 6 fold in B.C.G., 1. 5 and 1. 2 fold in M. vaccae. 2.4 and 2. 1 fold in M. kansasii , and 5.2 and 3.5 fold in M. scrofulaceum. 2. The various Mycobacteria grew better in cholesterol supplemented Dubos medium than in ordinary Dubos medium: 1. 5-2.1 fold increases bacterial growth in M. tuberculosis were observed in cholesterol supplemented Dubos medium compared with that in Dubos medium within 1-4 weeks, 1. 5-1. 8 fold in B.C.G ., 1.6-1.8 fold in M. kansasii. 1.3-1.6 fold in M. vaccae, 1. 3-1. 5 fold in M. scrofulaceum. 3. In the requirement of the additional effects of bovine serum albumin on cholesterol supplemented medium. there was a 1.5-3 fold increases of bacterial growth in KI basal medium enriched by both cholesterol and bovine serum albumin when compared with that in KI basal medium itself or enriched by cholesterol or bovine serum albumin alone. Therefore, mycobacterial growth appeared to be increased by adding cholesterol or hyaluronic acid to the medium, especially, cholesterol with bovine serum albumin.