Genomics Inform.  2022 Mar;20(1):e6. 10.5808/gi.21073.

Hypothetical protein predicted to be tumor suppressor: a protein functional analysis

• Kader MA
• Ahammed A
• Khan MS
• Al Ashik SA
• Islam MS
• Hossain MU

• KMID: 2527949
• DOI: http://doi.org/10.5808/gi.21073

Abstract

Litorilituus sediminis is a Gram-negative, aerobic, novel bacterium under the family of Colwelliaceae, has a stunning hypothetical protein containing domain called von Hippel-Lindau that has significant tumor suppressor activity. Therefore, this study was designed to elucidate the structure and function of the biologically important hypothetical protein EMK97_00595 (QBG34344.1) using several bioinformatics tools. The functional annotation exposed that the hypothetical protein is an extracellular secretory soluble signal peptide and contains the von Hippel-Lindau (VHL; VHL beta) domain that has a significant role in tumor suppression. This domain is conserved throughout evolution, as its homologs are available in various types of the organism like mammals, insects, and nematode. The gene product of VHL has a critical regulatory activity in the ubiquitous oxygen-sensing pathway. This domain has a significant role in inhibiting cell proliferation, angiogenesis progression, kidney cancer, breast cancer, and colon cancer. At last, the current study depicts that the annotated hypothetical protein is linked with tumor suppressor activity which might be of great interest to future research in the higher organism.

Keyword

functional annotation; hypothetical protein; novel bacterium; tumor suppressor; VHL domain