Korean J Urol Oncol.  2022 Feb;20(1):52-58. 10.22465/kjuo.2022.20.1.52.

Clinical Significance of Rab27a as a Urinary Biomarker in Patients With Bladder Cancer

• Ku JY
• Hwang EC
• Lee CH
• Kim KH
• Kwon DD
• Ha HK

• KMID: 2527251
• DOI: http://doi.org/10.22465/kjuo.2022.20.1.52

Abstract

Purpose
The aims of this study were to investigate the clinical value of Rab27a as a urinary biomarker, and its efficiency in the prediction of bladder cancer grade.
Materials and Methods
The expression of Rab27a in urine samples of patients with bladder cancer, cell line (T-24), and tissue samples of patients with bladder cancer was estimated via quantitative reverse transcription polymerase chain reaction (qRT-PCR). The Rab27a expression level was investigated according to sex, age, and histological grade via qRT-PCR and Western blotting.
Results
Rab27a was also expressed at high levels in urine compared to cell lines and tissues from bladder cancer patients. In addition, Rab27a expression varied significantly according to tumor grade (p<0.001). Rab27a was expressed at high levels in male and elderly patients, however, there was not statistically significant.
Conclusions
Our results indicated that Rab27a is valuable as a urinary diagnostic biomarker for bladder cancer. In addition, it may serve as a predictive factor for determining bladder cancer grade.

Keyword

Biomarker; Liquid biopsy; Rab27a; Urinary bladder neoplasms; Urine