J Dent Rehabil Appl Sci.  2021 Mar;37(1):31-38. 10.14368/jdras.2021.37.1.31.

Heme effects of hemin on growth of peridontopathogens

• Yoo HJ
• Lee SH

• KMID: 2525976
• DOI: http://doi.org/10.14368/jdras.2021.37.1.31

Abstract

Purpose
The purpose of this study was to investigate effect of heme on periodontopathogens. Materials and Methods: The experiment was performed using 7 types of anaerobic bacteria present in the periodontal pocket. The bacteria were cultured using suitable medium in an anaerobic condition with or without hemin, and the growth of the bacteria was measured every 6 hours by a spectrophotometer.
Results
the growth of Porphyromonas gingivalis was different only by the presence or absence of hemin. The growth of other periodontopathogens except Treponema denticola was different in a hemin concentration-dependent manner. The growth of T. denticola was interfered by hemin.
Conclusion
Heme may be a factor that leads dysbiosis in the microbial ecosystem of the subgingival plaque and thereby promote a periodontitis-causing environment.

Keyword

hemin; periodontopathogen; dysbiosis; growth

Figure

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  Fig. 1 Effect of hemin concentration on the growth of P. gingivalis. P. gingivalis was cultured with various concentration of hemin or without hemin. Asterisk (*) indicates statistically significant differences compared with hemin free (P < 0.05).

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  Fig. 2 Effect of hemin concentration on the growth of P. nigrescens. P. nigrescens was cultured with various concentration of hemin or without hemin. Asterisk (*) indicates statistically significant differences compared with hemin free (P < 0.05).

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  Fig. 3 Effect of hemin concentration on the growth of P. intermedia. P. intermedia was cultured with various concentration of hemin or without hemin. Asterisk (*) indicates statistically significant differences compared with hemin free (P < 0.05).

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  Fig. 4 Effect of hemin concentration on the growth of F. nucleatum. F. nucleatum was cultured with various concentration of hemin or without hemin. Asterisk (*) indicates statistically significant differences compared with hemin free (P < 0.05).

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  Fig. 5 Effect of hemin concentration on the growth of F. alocis. F. alocis was cultured with various concentration of hemin or without hemin. Asterisk (*) indicates statistically significant differences compared with hemin free (P < 0.05).

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  Fig. 6 Effect of hemin concentration on the growth of T. forsythia. T. forsythia was cultured with various concentration of hemin or without hemin. Asterisk (*) indicates statistically significant differences compared with hemin free (P < 0.05).

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  Fig. 7 Effect of hemin concentration on the growth of T. denticola. T. denticola was cultured with various concentration of hemin or without hemin. Asterisk (*) indicates statistically significant differences compared with hemin free (P < 0.05).

Reference

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