Clin Exp Vaccine Res.  2021 Sep;10(3):259-263. 10.7774/cevr.2021.10.3.259.

The effect of apolipoprotein A-I and perfluorocarbon emulsion on the production of specific antibodies in mice

• Zhalimov V
• Gritsyna Y
• Morenkov O

• KMID: 2525826
• DOI: http://doi.org/10.7774/cevr.2021.10.3.259

Abstract

Adjuvants are often required to improve the potentially low immunogenicity of vaccines. In this study, it is proposed to use an emulsion based on fluorocarbons as an adjuvant. Since this emulsion adsorbs only a small range of proteins, apolipoprotein A-I (ApoAI) was used as an anchor. Antigen and ApoAI were combined by creating a fusion construct. Results showed that the combined use of a perfluorocarbon emulsion and ApoAI during immunization significantly increases the specific antibody titer in mice and in its effectiveness this system is close to the incomplete Freund's adjuvant.

Keyword

Adjuvants; Perfluorocarbons; Apolipoprotein A-I; Adsorption; Synthetic biology