J Cancer Prev.  2021 Dec;26(4):304-308. 10.15430/JCP.2021.26.4.304.

Epithelial Cell-specific Deletion of Microsomal Prostaglandin E Synthase-1 Does Not Influence Colon Tumor Development in Mice

• Nakanishi M
• Rosenberg DW

• KMID: 2524125
• DOI: http://doi.org/10.15430/JCP.2021.26.4.304

Abstract

Activation of the COX-2/microsomal prostaglandin E synthase-1 (mPGES-1)/prostaglandin E ₂ (PGE ₂ ) signaling axis is a hallmark of many cancers, including colorectal cancer, prompting the implementation of prevention strategies targeting COX-2 activity. We have previously shown that targeting the downstream terminal PGE ₂  synthase, mPGES-1 (Ptges), specifically reduces inducible PGE ₂  formation without disrupting synthesis of other essential prostanoids, thereby conferring dramatic cancer protection against colon carcinogenesis in multiple mouse models. In order to accelerate its development as a viable drug target, and to better understand the mechanisms by which PGE ₂ influences colon carcinogenesis, we recently developed a conditional Ptges knockout mouse model (cKO). To evaluate the functional role of Ptges directly within the colonic epithelia, cKO mice were crossed with carbonic anhydrase 1 (Car1)-Cre mice (cKO.Car1), and colon tumors were induced using the azoxymethane/dextran sodium sulfate protocol. Unexpectedly, epithelial-specific blockade of Ptges failed to protect mice against colon tumor development. Further studies using the cKO mouse model will be necessary to pinpoint the cell type-specific location of mPGES-1 and its control of inducible PGE ₂ formation that drives tumor formation in the colon.

Keyword

PGE ₂; mPGES-1; Colonic neoplasms; Azoxymethane; Dextran sulfate sodium