Korean J Parasitol.
2021 Dec;59(6):615-623. 10.3347/kjp.2021.59.6.615.
Proteomic and Immunological Identification of Diagnostic Antigens from Spirometra erinaceieuropaei Plerocercoid
- Affiliations
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- 1National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research); WHO Collaborating Center for Tropical Diseases; NHC Key Laboratory of Parasite and Vector Biology (National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention); National Center for International Research on Tropical Diseases; Shanghai, P. R. China
- 2The Third Hospital of Shijiazhuang City, Shijiazhuang, P. R. China
- 3School of Public Health, Weifang Medical University, Weifang, P. R. China
- KMID: 2523533
- DOI: http://doi.org/10.3347/kjp.2021.59.6.615
Abstract
- Human sparganosis is a food-borne parasitic disease caused by the plerocercoids of Spirometra species. Clinical diagnosis of sparganosis is crucial for effective treatment, thus it is important to identify sensitive and specific antigens of plerocercoids. The aim of the current study was to identify and characterize the immunogenic proteins of Spirometra erinaceieuropaei plerocercoids that were recognized by patient sera. Crude soluble extract of the plerocercoids were separated using 2-dimensional gel electrophoresis coupled with immunoblot and mass spectrometry analysis. Based on immunoblotting patterns and mass spectrometry results, 8 antigenic proteins were identified from the plerocercoid. Among the proteins, cysteine protease protein might be developed as an antigen for diagnosis of sparganosis.
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