J Breast Cancer.
2021 Oct;24(5):463-473. 10.4048/jbc.2021.24.e40.
Longitudinal Intravital Imaging of Tumor-Infiltrating Lymphocyte Motility in Breast Cancer Models
- Park I
1,2 - Hong S3,4
- Seok J5
- Lucia SE4,5
- Song E3
- Kim M6
- Kong E4,5
- Seo H3,4
- Hwang Y3,4
- Ahn S3,4
- Kim S1
- Jang DH1,2
- Lee JH1,2
- Park SH5
- Kim P3,4,5
- Jo YH1,2
- Affiliations
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- 1Department of Emergency Medicine, Seoul National University Bundang Hospital (SNUBH), Seongnam, Korea
- 2Department of Emergency Medicine, Seoul National University College of Medicine, Seoul, Korea
- 3Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea
- 4KI for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea
- 5Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea
- 6Division of Rheumatology, Department of Internal Medicine, Gyeongsang National University School of Medicine, Jinju, Korea
- KMID: 2522097
- DOI: http://doi.org/10.4048/jbc.2021.24.e40
Abstract
- Immunoreactive dynamics of tumor-infiltrating lymphocytes (TILs) within the tumor microenvironment in breast cancer are not well understood. This study aimed to investigate the spatiotemporal cellular dynamics of TILs in breast cancer models. Breast cancer cells were implanted into the dorsal skinfold chamber of BALB/c nude mice, and T lymphocytes were adoptively transferred. Longitudinal intravital imaging was performed, and the spatiotemporal dynamics of TILs were assessed. In the 4T1 model, TILs progressively exhibited increased motility, and their motility inside the tumor was significantly higher than that outside the tumor. In the MDA-MB-231 model, the motility of TILs progressively decreased after an initial increase. TIL motility in the MDA-MB-231 and MCF-7 models differed significantly, suggesting an association between programmed death-ligand 1 expression levels and TIL motility, which warrants further investigation. Furthermore, intravital imaging of TILs can be a useful method for addressing dynamic interactions between TILs and breast cancer cells.
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