Int J Stem Cells.  2021 May;14(2):138-149. 10.15283/ijsc20169.

Organoids: A New Model for SARS-CoV-2 Translational Research

• Yu J

• KMID: 2515991
• DOI: http://doi.org/10.15283/ijsc20169

Abstract

The 2019-novel coronavirus (SARS-CoV-2) pneumonia epidemic is a thorny public health problem faced by health officials and a major cause of concern for health professionals. However, the currently used immortalized cell lines and animal models, though easy to manipulate, can not thoroughly simulate real viral activity due to a lack of target cells, species isolation, and insufficient adequate tissues and organs for clinical research. Organoid that emerges as an effective model and time-saving approach can simulate the viral life cycle in vitro and explore a therapeutic target for antiviral drug development. The 3D tissue cultures contain patient-specific stem cells in vitro to mimic the complexity of real tissue within the 3D microstructure that has the same functionality as the tissue of interest. It avoids the problems such as the distortion of genetic markers and animal ethics of using 2D cultures for animal testing and can be employed in studies of specific-organ viral infections to fully understand the physiopathological mechanism of SARS-CoV-2 infection for vaccine research and development.

Keyword

Coronavirus 2019 disease; Stem cells; Organoids; Translational research

Figure

• 

  Fig. 1 Several organoids have been established to study SARS-CoV-2. The upper refers to clinical symptoms of different organs infected by COVID-19, the medium is mainly emphasised on the mechanism of Inflammatory cytokine cascade after virus infection, and then, the bottom is now there are seveal organoids models (1. Brain organoids; 2. Heart organoids; 3. Lung organoids; 4. Gastrointestinal organoids; 5. Kidney organoids) used to screen corres-ponding anti-virual drugs, further-more, organoids-on-the-Chips is a more complete model of studying the entire process of virus infection in next days.

Reference

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