Tissue Eng Regen Med.  2020 Dec;17(6):787-800. 10.1007/s13770-020-00311-2.

Microfluidic Systems for Assisted Reproductive Technologies: Advantages and Potential Applications

• Sequeira RC
• Criswell T
• Atala A
• Yoo JJ

• KMID: 2509424
• DOI: http://doi.org/10.1007/s13770-020-00311-2

Abstract

Microfluidic technologies have emerged as a powerful tool that can closely replicate the in-vivo physiological conditions of organ systems. Assisted reproductive technology (ART), while being able to achieve successful outcomes, still faces challenges related to technical error, efficiency, cost, and monitoring/assessment. In this review, we provide a brief overview of the uses of microfluidic devices in the culture, maintenance and study of ovarian follicle development for experimental and therapeutic applications. We discuss existing microfluidic platforms for oocyte and sperm selection and maintenance, facilitation of fertilization by in-vitro fertilization/intracytoplastimc sperm injection, and monitoring, selection and maintenance of resulting embryos. Furthermore, we discuss the possibility of future integration of these technologies onto a single platform and the limitations facing the development of these systems. In spite of these challenges, we envision that microfluidic systems will likely evolve and inevitably revolutionize both fundamental, reproductive physiology/toxicology research as well as clinically applicable ART.

Keyword

Microfluidics; In-vitro oocyte culture; In-vitro sperm culture; Assisted reproductive technology; Ovarian follicle development