J Korean Acad Oral Health.  2020 Sep;44(3):126-129. 10.11149/jkaoh.2020.44.3.126.

Caries activity test by realtime PCR method and bacterial cultural method

Affiliations
  • 1Department of Oral Health, Graduate School of Public Health and Social Welfare, Dankook University, Cheonan, Korea
  • 2Department of Dental Hygiene, Gangneung Yeongdong University, Gangneung, Korea
  • 3Department of Preventive Dentistry, College of Dentistry, Dankook University, Cheonan, Korea

Abstract


Objectives
This study aimed to compare the results of conventional methodology and real-time polymerase chain reaction (PCR) for acid-producing bacteria and identify the appropriate method for caries activity testing.
Methods
Oral examination and caries activity tests, such as the CRT® Bacteria test and modified Snyder test, were conducted on 33 middle school students in Cheongju, South Korea. Pearson correlation analysis was subsequently performed with a significance level of 5%.
Results
The amount of Streptococcus mutans determined using the CRT® Bacteria test was highly correlated with the decayed teeth (DT) index (R=0.570, P<0.05); decayed, missing, and filled teeth (DMFT) index (R=0.376, P<0.05); and dental health capacity of the first permanent molar (R=―0.395, P<0.05). The amount of Streptococcus mutans determined using real-time PCR was significantly associated with the DT index (R=0.528, P<0.05), DMFT index (R=0.369, P<0.05), and dental health capacity of the first permanent molar (R=―0.426, P=0.013).
Conclusions
There was a significant correlation between the amount and the type of bacteria between the CRT® Bacteria test and real-time PCR; therefore, these tests were found to be more appropriate to determine caries activity than the modified Snyder test. This study suggests that realtime PCR is a better technique for detecting caries activities than conventional techniques, such as the CRT® Bacteria test and modified Snyder test, because it is easy to use and provides accurate results.

Keyword

Caries activity test; Oral microorganism; Streptococcus mutans
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