Investig Clin Urol.  2020 May;61(3):297-303. 10.4111/icu.2020.61.3.297.

Expression of bladder α1-adrenoceptor subtype after relief of partial bladder outlet obstruction in a rat model

• Lee JY
• Park JM
• Na YG
• Song KH
• Lim JS
• Yang SW
• Kim GH
• Shin JH

• KMID: 2500584
• DOI: http://doi.org/10.4111/icu.2020.61.3.297

Abstract

Purpose
Many patients with benign prostatic hyperplasia require treatment for persistent storage symptoms, even when the obstruction is successfully relieved by surgery. Previous studies identified a characteristic increase in α1D-adrenoceptor levels in the bladder in a bladder outlet obstruction (BOO) model. Here, we investigated the expression of α1-adrenoceptor subtypes in the bladder after relief of partial BOO (pBOO) in a rat model.
Materials and Methods
A total of 60 female Sprague–Dawley rats were randomly divided into three groups (sham-operated, pBOO, and pBOO relief groups), and the expression of α1-adrenoceptor subtypes in the urothelium and detrusor muscle tissues was examined by western blot.
Results
The expression of the α1D-adrenoceptor was significantly higher in the urothelium and detrusor muscle tissue of the pBOO and pBOO relief groups than in the corresponding tissue of the sham-operated group. Additionally, the α1A-adrenoceptor was predominant in the sham-operated group but significantly decreased in the urothelium in the pBOO group. No significant differences were found in α1A-adrenoceptor levels in detrusor muscle or whole bladder.
Conclusions
Our results showed that α1D-adrenoceptor levels were consistently increased with pBOO, even after relief, suggesting that the α1D-adrenoceptor might be a cause of persistent storage symptoms after relief of pBOO.

Keyword

Adrenergic alpha-1 receptor antagonists; Lower urinary tract symptoms; Urinary bladder neck obstruction; Urinary bladder, overactive