J Korean Med Sci.  2020 Feb;35(7):e84. 10.3346/jkms.2020.35.e84.

Virus Isolation from the First Patient with SARS-CoV-2 in Korea

Affiliations
  • 1Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea. mdohmd@snu.ac.kr
  • 2Laboratory of Infection & Immunity, Seoul National University Hospital Biomedical Research Institute, Seoul, Korea.
  • 3Macrogen Inc., Seoul, Korea.
  • 4Department of Internal Medicine, Incheon Medical Center, Incheon, Korea.
  • 5Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.
  • 6Precision Medicine Center, Seoul National University Bundang Hospital, Seongnam, Korea.

Abstract

Novel coronavirus (SARS-CoV-2) is found to cause a large outbreak started from Wuhan since December 2019 in China and SARS-CoV-2 infections have been reported with epidemiological linkage to China in 25 countries until now. We isolated SARS-CoV-2 from the oropharyngeal sample obtained from the patient with the first laboratory-confirmed SARS-CoV-2 infection in Korea. Cytopathic effects of SARS-CoV-2 in the Vero cell cultures were confluent 3 days after the first blind passage of the sample. Coronavirus was confirmed with spherical particle having a fringe reminiscent of crown on transmission electron microscopy. Phylogenetic analyses of whole genome sequences showed that it clustered with other SARS-CoV-2 reported from Wuhan.

Keyword

2019 Novel Coronavirus; Culture; Microscopy, Electron; Phylogeny; Korea

MeSH Terms

China
Coronavirus
Crowns
Genome
Humans
Korea*
Microscopy, Electron
Microscopy, Electron, Transmission
Phylogeny
Vero Cells
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