J Cancer Prev.  2019 Sep;24(3):155-162. 10.15430/JCP.2019.24.3.155.

Involvement of Up-regulation of Death Receptors and Bim in Hispolon-mediated TNF-related Apoptosis-inducing Ligand Sensitization in Human Renal Carcinoma

• Yun JM
• Min KJ
• Kwon TK

• KMID: 2468797
• DOI: http://doi.org/10.15430/JCP.2019.24.3.155

Abstract

BACKGROUND
Hispolon has been shown to possess antitumor effects in various cancer cells. However, the underlying mechanisms are not fully understood. In this study, we evaluated the sensitizing effect of hispolon on TNF-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis in human renal carcinoma cells.
METHODS
Apoptosis was analyzed by using cell-based cytometer. The mRNA levels were assessed by reverse transcription-PCR. Bax activation was determined by oligomerization and fluorescence-activated cell sorting with Bax-NT monoclonal antibody. The protein expression was measured by Western blotting.
RESULTS
Hispolon induced up-regulation of Bim and death receptors expression at the post-translational level.
CONCLUSIONS
Hispolon enhanced TRAIL-mediated apoptosis in renal carcinoma cells, but not in normal cells.

Keyword

Hispolon; TNF-related apoptosis-inducing ligand; Apoptosis; Death receptors; Bim

MeSH Terms

Apoptosis
Blotting, Western
Flow Cytometry
Humans*
Receptors, Death Domain*
RNA, Messenger
TNF-Related Apoptosis-Inducing Ligand*
Up-Regulation*
RNA, Messenger
Receptors, Death Domain
TNF-Related Apoptosis-Inducing Ligand