Yonsei Med J.  2016 Jan;57(1):203-208. 10.3349/ymj.2016.57.1.203.

Development of a Synthetic Surfactant Using a Surfactant Protein-C Peptide Analog: In Vitro Studies of Surface Physical Properties

Affiliations
  • 1Department of Pediatrics, Kyung Hee University School of Medicine, Seoul, Korea. baecw@khnmc.or.kr

Abstract

PURPOSE
Pulmonary surfactant (PS) replacement has been the gold standard therapy for neonatal respiratory distress syndrome; however, almost all commercial PSs contain animal proteins. We prepared a synthetic PS by using a human surfactant protein (SP) analog and evaluated its in vitro properties.
MATERIALS AND METHODS
A peptide sequence (CPVHLKRLLLLLLLLLLLLLLLL) of human SP-C was chosen to develop the peptide analog (SPa-C). The new synthetic SP-C PS (sSP-C PS) was synthesized from SPa-C, dipalmitoyl phosphatidylcholine, phosphatidyl glycerol, and palmitic acid. Physical properties of the sSP-C PS were evaluated by measuring the maximum and minimum surface tensions (STs), surfactant spreading, and adsorption rate. In addition, we recorded an ST-area diagram. The data obtained on sSP-C PS were subsequently compared with those of purified natural bovine surfactant (PNBS), and the commercial product, Surfacten(R).
RESULTS
The sSP-C PS and Surfacten(R) were found to have maximum ST values of 32-33 mN/m, whereas that of PNBS was much lower at 19 mN/m. The minimum ST values of all three products were less than 10 mN/m. The values that were measured for the equilibrium ST of rapidly spreading sSP-C PS, Surfacten(R), and PNBS were 27, 27, and 24 mN/m, respectively. The surface adsorptions were found to be the same for all three PSs (20 mN/m). ST-area diagrams of sSP-C PS and Surfacten(R) revealed similar properties.
CONCLUSION
In an in vitro experiment, the physical properties exhibited by sSP-C PS were similar to those of Surfacten(R). Further study is required to evaluate the in vivo efficacy.

Keyword

Pulmonary surfactant; surfactant protein-C; synthetic peptide syntheses; respiratory distress syndrome; surface tension

MeSH Terms

1,2-Dipalmitoylphosphatidylcholine/analogs & derivatives
Adsorption
Amino Acid Sequence/*genetics
Animals
C-Peptide/*chemistry
Cattle
Humans
Infant, Newborn
Pulmonary Surfactant-Associated Protein C/*chemical synthesis/pharmacology
Pulmonary Surfactants/*chemical synthesis/pharmacology
Respiratory Distress Syndrome, Newborn/*drug therapy
*Surface Properties
*Surface Tension
Surface-Active Agents
C-Peptide
1,2-Dipalmitoylphosphatidylcholine
Pulmonary Surfactant-Associated Protein C
Pulmonary Surfactants
Surface-Active Agents
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