J Gynecol Oncol.  2019 Mar;30(2):e26. 10.3802/jgo.2019.30.e26.

Olaparib induced senescence under P16 or P53 dependent manner in ovarian cancer

Affiliations
  • 1Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China. liuhaiou@fudan.edu.cn
  • 2Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, China.
  • 3Department of Health Science, Graduate School of Medical, Osaka University, Osaka, Japan.
  • 4Major of Biotechnological Pharmaceutics, Shanghai Pharmaceutical School, Shanghai, China.
  • 5Department of Obstetrics and Gynecology, Fudan University Shanghai Medical College, Shanghai, China.

Abstract


OBJECTIVE
Poly (ADP-ribose) polymerase (PARP) is an important molecule in the early stress response of DNA damage, which is involved in DNA damage repair and cellular senescence. Olaparib, as PARP inhibitor, has an anti-tumor effect on high grade serous ovarian cancer, but its effects on cellular senescence have not been reported. This study intends to explore the role of olaparib in the regulation of senescence in ovarian cancer cells.
METHODS
The effects of olaparib on the senescence of ovarian cancer cells were detected by using the senescence-associated β-galactosidase (SA-β-Gal) and senescence-associated heterochromatin aggregation (SAHF). Quantitative real-time polymerase chain reaction was used to analyze the senescence-associated secretory phenotype (SASP). Cell cycle and apoptosis were detected by flow cytometry. The effect of olaparib on tumor growth was analyzed in a nude mouse xenograft transplantation model.
RESULTS
Long-term (6 days) treatment with olaparib (5 μM) significantly inhibited the growth of ovarian cancer cells, leading to arrest the cell cycle at G0/G1 phase, significant increase the number of positive SA-β-Gal stained cells and positive SAHF cells. The expression of P16 and retinoblastoma protein (p-RB) were significantly enhanced in SKOV3 cells under olaparib treated, meanwhile, the expression of P53 and p-RB were upregulated in A2780 cells. In OVCAR-3 cells, the expression of P53 was downregulated and p-RB was upregulated. Mice with SKOV3 xenograft transplantation was given olaparib (10 mg/kg/day) via abdominal cavity administration, the tumor volume was reduced (p < 0.01).
CONCLUSION
Continuous low dosage administration of olaparib induced senescence under P16 or P53 dependent manner in ovarian cancer.

Keyword

Olaparib; Senescence; Ovarian Neoplasms

MeSH Terms

Abdominal Cavity
Aging*
Animals
Apoptosis
Cell Aging
Cell Cycle
DNA Damage
Flow Cytometry
Heterochromatin
Mice
Mice, Nude
Ovarian Neoplasms*
Phenotype
Real-Time Polymerase Chain Reaction
Retinoblastoma Protein
Transplantation, Heterologous
Tumor Burden
Heterochromatin
Retinoblastoma Protein
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