Nutr Res Pract.  2019 Jun;13(3):189-195. 10.4162/nrp.2019.13.3.189.

Anti-inflammatory effect of aged black garlic on 12-O-tetradecanoylphorbol-13-acetate-induced dermatitis in mice

Affiliations
  • 1Department of Food and Nutrition, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea. mrkim@cnu.ac.kr
  • 2Korean Medicine-Application Center, Korea Institute of Oriental Medicine, Daegu 41062, Korea.

Abstract

BACKGROUND/OBJECTIVES
Although aged black garlic has various biological activities such as anti-allergy, anti-inflammation and neuroprotection, effect of aged black garlic on chemically contact dermatitis is unclarified.
MATERIALS/METHODS
To evaluate anti-dermatitic activity of aged black garlic extract, we investigated effects of a fraction of aged black garlic extract (BG10) on both in vivo and in vitro.
RESULTS
BG10 almost inhibited formation of nitric monoxide and interleukin-6 (IL-6; IC50, 7.07 µg/mL) at 25 µg/mL, and dose-dependently reduced production of tumor necrosis factor-α (TNF-α; IC50, 52.07 µg/mL) and prostaglandin E2 (IC50, 38.46 µg/mL) in lipopolysaccharide-stimulated RAW264.7 cells. In addition, BG10 significantly inhibited the expression of inducible nitric oxide synthase, cyclooxygenase-2 and nuclear NF-κB, and improved that of cytosolic levels of NF-κB and IκBα in the cells. Consistent with in vitro studies, BG10 (0.5 mg/mL) not only reduced ear edema but also suppressed the formation of IL-6 and TNF-α induced by 12-O-tetradecanoylphorbol-13-acetate in ear tissues of mice.
CONCLUSIONS
These findings suggest BG10 has anti-dermatitic activity through inhibiting activation of macrophages. Therefore, such effects of BG10 may provide information for the application of aged black garlic for prevention and therapy of contact dermatitis.

Keyword

Contact dermatitis; COX-2; cytokines; macrophage; NF-kappa B

MeSH Terms

Animals
Cyclooxygenase 2
Cytokines
Cytosol
Dermatitis*
Dermatitis, Contact
Dinoprostone
Ear
Edema
Garlic*
In Vitro Techniques
Inhibitory Concentration 50
Interleukin-6
Macrophages
Mice*
Necrosis
Neuroprotection
NF-kappa B
Nitric Oxide Synthase Type II
Cyclooxygenase 2
Cytokines
Dinoprostone
Interleukin-6
NF-kappa B
Nitric Oxide Synthase Type II
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