J Dent Hyg Sci.  2019 Jun;19(2):107-112. 10.17135/jdhs.2019.19.2.107.

Effect of Oral Spray on Dental Plaque Bacteria and Oral Epithelial Cells

  • 1Department of Dental Hygiene, College of Health Science, Eulji University, Seongnam 13135, Korea. kiteys@eulji.ac.kr


Good oral health is important for systemic body health and quality of life. Spray oral cleansers are increasingly preferred because of their convenience of carrying and the ease of oral hygiene management. In addition, many kinds of oral cleanser products containing various ingredients with antibacterial, washing, and moisturizing effects are being manufactured. However, concerns about the safety and side effects of oral sprays are increasing, and there is very little information regarding the use and care of oral sprays is available to consumers. This study aimed to investigate the effects of oral spray on oral bacteria and tissue to elucidate the factors that need to be considered when using oral sprays.
The effects of oral spray on the growth of dental plaque bacteria was assessed using disk diffusion assays. Cytotoxicity and morphological changes in oral epithelial cells were observed by microscopy. The effects of oral spray on dental plaque growth were also confirmed on specimens from permanent incisors of bovines by Coomassie staining.
The pH of spray products, such as Perioe Dental Cooling, Cool Sense, and Dentrix, were 3.65, 3.61, and 6.15, respectively. All tested spray products showed strong toxicity to dental plaque bacteria and oral epithelial cells. Compared with those on the control, dental plaque bacteria deposits on the enamel surface increased following the use of oral spray.
Three types of oral spray, namely Perioe Dental Cooling, Cool Sense, and Dentrix, strongly inhibited the growth of dental plaque bacteria and oral epithelial cells. The oral spray ingredient enhanced dental plaque growth on the enamel surface. Users should be informed of precautions when using oral sprays and the need for oral hygiene after its use.


Bacteria; Cytotoxicity; Oral hygiene; Oral sprays; Plague

MeSH Terms

Dental Enamel
Dental Plaque*
Epithelial Cells*
Hydrogen-Ion Concentration
Oral Health
Oral Hygiene
Oral Sprays
Quality of Life
Oral Sprays
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