Int J Stem Cells.  2019 Mar;12(1):107-113. 10.15283/ijsc18101.

Possibility of Injecting Adipose-Derived Stromal Vascular Fraction Cells to Accelerate Microcirculation in Ischemic Diabetic Feet: A Pilot Study

• Moon KC
• Chung HY
• Han SK
• Jeong SH
• Dhong ES

• KMID: 2447229
• DOI: http://doi.org/10.15283/ijsc18101

Abstract

BACKGROUND AND OBJECTIVES
Beneficial effects of human adipose-derived stromal vascular fraction (SVF) cell injection on microcirculation have been recently reported in in vitro and in vivo studies. However, no clinical studies have reported its effect in diabetic patients who commonly experience compromised tissue perfusion, regardless of the status of intravascular blood flow. The present piloting study was designed to clinically examine the possibility of SVF cell injection to accelerate microcirculation, particularly in ischemic diabetic feet.
METHODS
Ten diabetic feet were included to receive subcutaneous injection of SVF cells around wounds. Transcutaneous partial oxygen pressure (TcPO2) and cutaneous microvascular blood flow were measured before and every four weeks after cell injection until the 12th week visit.
RESULTS
TcPO2 values increased from 31.3±7.4 before injection to 46.4±8.2 mmHg at 12 weeks after SVF injection (1.5-fold, p<0.05). Cutaneous microvascular blood flow levels increased from 34.0±21.1 before injection to 76.1±32.5 perfusion unit at 12 weeks after SVF injection (2.2-fold, p<0.05). There were no adverse events related to SVF cell injection.
CONCLUSIONS
Results of this study demonstrate that adipose-derived SVF cell injection have the possibility to provide beneficial effects on microcirculation in ischemic diabetic feet.

Keyword

Diabetic foot; Microcirculation; Stromal vascular fraction cell; Tissue oxygenation

MeSH Terms

Diabetic Foot*
Humans
In Vitro Techniques
Injections, Subcutaneous
Microcirculation*
Oxygen
Perfusion
Pilot Projects*
Wounds and Injuries
Oxygen

Figure

• 

  Fig. 1 SVF cell isolation and injection. (A) The aspirated adipose tissue was prepared and introduced into a processing chamber with a single-use sterile disposable set. (B) SVF cells were collected in a processing canister. (C) SVF cells in the canister were aspirated using a 10-cc syringe immediately prior to injection and (D) injected into subcutaneous layers around the diabetic wound using a 23-gauge needle.

• 

  Fig. 2 Sequential values of TcPO2 and cutaneous microvascular blood flow (*p<0.05).

Reference

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