J Vet Sci.  2019 Jan;20(1):91-94. 10.4142/jvs.2019.20.1.91.

Genetic identification of astroviruses in wild boars

• Vilcek S
• Salamunova S
• Jackova A

• KMID: 2434783
• DOI: http://doi.org/10.4142/jvs.2019.20.1.91

Abstract

Astroviruses are widely detected in pigs but their detection in wild boars is rather sporadic. In this study, astroviruses were detected in organ homogenates of wild boars by applying nested reverse transcriptase polymerase chain reaction, and the typing was carried out by phylogenetic analysis. Overall, 30/200 (15.0%) homogenates were positive for astroviruses. Genetic typing revealed that of 13 amplicons analyzed, 8 were typed as porcine astrovirus lineage 2 (PAstV-2), 2 as lineage 4 (PAstV-4), 2 identical sequences were grouped with chicken astrovirus, and 1 sequence belonged to a bat astrovirus lineage. This first identification of chicken and bat astroviruses in wild boars indicates interspecies transmission.

Keyword

Wild boar; Porcine astrovirus; Chicken astrovirus; Bat astrovirus; Phylogenetic analysis

MeSH Terms

Avastrovirus
Chickens
Reverse Transcriptase Polymerase Chain Reaction
Sus scrofa*
Swine

Figure

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  Fig. 1 Phylogenetic tree of astroviruses. The tree was constructed from a 317 to 347 bp DNA fragment (primers were omitted) depending on the astrovirus nucleotide sequences of the RNA-dependent RNA polymerase (RdRp) gene by a neighbor-joining method incorporated in MEGA6 [13]. Sequences from Slovakian wild boar samples are in bold with arrows, sequences from domestic pigs in Slovakia are marked with a triangle, while those from chickens are marked with a black circle and from pheasants with a black cube. Selected sequences from GenBank are labeled with their country of origin and accession numbers. The scale bar represents the number of nucleotide substitutions per site. PAstV, porcine astrovirus; CAstV, chicken astrovirus; TAstV, Turkey astrovirus; BAstV, bat astrovirus; DAstV, duck astrovirus; ANV, avian nephritis virus.

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