Blood Res.  2018 Sep;53(3):251-254. 10.5045/br.2018.53.3.251.

Massive splenomegaly: flow cytometry as a diagnostic tool for systemic mastocytosis

• Rastogi L
• Dass J
• Dhingra G
• Gupta N
• Kotwal J

• KMID: 2429327
• DOI: http://doi.org/10.5045/br.2018.53.3.251

Abstract

No abstract available.

MeSH Terms

Flow Cytometry*
Mastocytosis, Systemic*
Splenomegaly*

Figure

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  Fig. 1 (A) Toluidine blue staining shows mast cells in a marrow particle (toluidine blue, ×200). The inset shows spindle cell morphology of mast cells (H&E, ×400). (B) CD45 vs side scatter plot (SSC) to gate lymphocytes (reddish brown). (C) Gating for mast cells was done on CD117 vs SSC dot plot. Bright CD117+ cells are gated as mast cells (dark blue). (D, E) Dot plots and color density plots respectively showing an aberrant expression of CD2 and CD25 on the mast cell population. The intensity of CD2 is lesser than the normal T lymphocytes.

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  Fig. 2 (A) Paratrabecular mast cell aggregate showing new bone formation (H&E, ×100). (B) A high-power view shows the atypical morphology of mast cells with a predominance of oval- and spindle-shaped mast cells. Eosinophils are interspersed in the aggregates (H&E, ×400). (C) Toluidine blue staining shows metachromasia in mast cell aggregates (×200). (D) CD117 immunohistochemistry on bone marrow biopsy is positive in paratrabecular mast cell aggregates (×200).

Reference

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