Ann Lab Med.  2018 Nov;38(6):619-622. 10.3343/alm.2018.38.6.619.

Characterizing Atypical BCL6 Signal Patterns Detected by Digital Fluorescence In Situ Hybridization (FISH) Analysis

• Liew M
• Rowe LR
• Szankasi P
• Paxton CN
• Kelley T
• Toydemir R
• Salama ME

• KMID: 2429133
• DOI: http://doi.org/10.3343/alm.2018.38.6.619

Abstract

No abstract available.

MeSH Terms

Fluorescence*
In Situ Hybridization*

Figure

• 

  Fig. 1 Results from the combined testing of sample 1U. (A) Next generation sequencing identified a BCL6-IGH fusion. (B) Representative cells with 1F/1G and 2F/1G signal patterns with the BCL6 probe set. (C) The molecular inversion probe array analysis detected a copy number gain from 3p14.2 to 3q27.3.

Reference

1. Albagli-Curiel O. Ambivalent role of BCL6 in cell survival and transformation. Oncogene. 2003; 22:507–516.

2. Ohno H, Fukuhara S. Significance of rearrangement of the BCL6 gene in B-cell lymphoid neoplasms. Leuk Lymphoma. 1997; 27:53–63.

3. Willis TG, Dyer MJ. The role of immunoglobulin translocations in the pathogenesis of B-cell malignancies. Blood. 2000; 96:808–822.

4. Martin-Subero JI, Gesk S, Harder L, Grote W, Siebert R. Interphase cytogenetics of hematological neoplasms under the perspective of the novel WHO classification. Anticancer Res. 2003; 23:1139–1148.

5. Vargas AC, Selinger CI, Satgunaseelan L, Cooper WA, Gupta R, Stalley P, et al. Atypical Ewing sarcoma breakpoint region 1 fluorescence in-situ hybridization signal patterns in bone and soft tissue tumours: diagnostic experience with 135 cases. Histopathology. 2016; 69:1000–1011.

6. Liew M, Rowe L, Clement PW, Miles RR, Salama ME. Validation of break-apart and fusion MYC probes using a digital fluorescence in situ hybridization capture and imaging system. J Pathol Inform. 2016; 7:20.

7. Paxton CN, Rowe LR, South ST. Streamlining the OncoScan array procedure for use in a clinical laboratory. J Assoc Genet Technol. 2015; 41:61–65.