J Vet Sci.  2018 Nov;19(6):846-849. 10.4142/jvs.2018.19.6.846.

Molecular phylogenetic studies on clinical bovine piroplasmosis caused by benign Theileria in Shaanxi Province, China

• Wang J
• Zhang J
• Zhu Z
• Zhou X
• Li B

• KMID: 2427034
• DOI: http://doi.org/10.4142/jvs.2018.19.6.846

Abstract

A group of benign Theileria species, which are often referred to as T. orientalis/T. buffeli/T. sergenti group, has low pathogenicity in cattle. Herein, we report on Theileria spp. in cattle on a farm from China. Based on phylogenetic analysis of the major piroplasm surface protein gene sequences, we detected 6 genotypes that were categorized as Types 1, 2, 3, 4, and 5 as well as an additional Type 9 genotype. The new epidemiological features of the T. orientalis/T. buffeli/T. sergenti parasites in China indicate a greater diversity in the genetics of these species than had been previously thought.

Keyword

MPSP gene; Theileria spp.; genotype; phylogeny

MeSH Terms

Agriculture
Animals
Babesiosis*
Cattle
China*
Genetics
Genotype
Parasites
Phylogeny
Theileria*
Virulence

Figure

• 

  Fig. 1 Giemsa-stained blood smear from an infected calf. The arrows point out different polymorphisms of Theileria orientalis. Scale bar = 10 µm.

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  Fig. 2 Phylogenetic relationships among Theileria isolated in Shaanxi Province, China, based on major piroplasm surface protein (MPSP) gene sequences. The bold font indicates sequences obtained from this study. Bootstrap values are shown as percentages at each node based on 1,000 replicates. Branch lengths correlate to the number of substitutions inferred according to the scale shown.

Reference

1. Gubbels MJ, Hong Y, van der Weide M, Qi B, Nijman IJ, Guangyuan L, Jongejan F. Molecular characterisation of the Theileria buffeli/orientalis group. Int J Parasitol. 2000; 30:943–952.

2. He L, Feng HH, Zhang WJ, Zhang QL, Fang R, Wang LX, Tu P, Zhou YQ, Zhao JL, Oosthuizen MC. Occurrence of Theileria and Babesia species in water buffalo (Bubalus babalis, Linnaeus, 1758) in the Hubei province, South China. Vet Parasitol. 2012; 186:490–496.

3. Jeong W, Yoon SH, An DJ, Cho SH, Lee KK, Kim JY. A molecular phylogeny of the benign Theileria parasites based on major piroplasm surface protein (MPSP) gene sequences. Parasitology. 2010; 137:241–249.

4. Kamau J, de Vos AJ, Playford M, Salim B, Kinyanjui P, Sugimoto C. Emergence of new types of Theileria orientalis in Australian cattle and possible cause of theileriosis outbreaks. Parasit Vectors. 2011; 4:22.

5. Khukhuu A, Lan DT, Long PT, Ueno A, Li Y, Luo Y, Macedo AC, Matsumoto K, Inokuma H, Kawazu S, Igarashi I, Xuan X, Yokoyama N. Molecular epidemiological survey of Theileria orientalis in Thua Thien Hue province, Vietnam. J Vet Med Sci. 2011; 73:701–705.

6. Kim SJ, Tsuji M, Kubota S, Wei Q, Lee JM, Ishihara C, Onuma M. Sequence analysis of the major piroplasm surface protein gene of benign bovine Theileria parasites in east Asia. Int J Parasitol. 1998; 28:1219–1227.

7. Kubota S, Sugimoto C, Onuma M. A genetic analysis of mixed population in Theileria sergenti stocks and isolates using allele-specific polymerase chain reaction. J Vet Med Sci. 1995; 57:279–282.

8. Liu Q, Zhou YQ, He GS, Oosthuizen MC, Zhou DN, Zhao JL. Molecular phylogenetic studies on Theileria spp. isolates (China) based on small subunit ribosomal RNA gene sequences. Trop Anim Health Prod. 2010; 42:109–114.

9. McFadden AM, Rawdon TG, Meyer J, Makin J, Morley CM, Clough RR, Tham K, Mullner P, Geysen D. An outbreak of haemolytic anaemia associated with infection of Theileria orientalis in naive cattle. N Z Vet J. 2011; 59:79–85.

10. Ota N, Mizuno D, Kuboki N, Igarashi I, Nakamura Y, Yamashina H, Hanzaike T, Fujii K, Onoe S, Hata H, Kondo S, Matsui S, Koga M, Matsumoto K, Inokuma H, Yokoyama N. Epidemiological survey of Theileria orientalis infection in grazing cattle in the eastern part of Hokkaido, Japan. J Vet Med Sci. 2009; 71:937–944.

11. Rishniw M, Barr SC, Simpson KW, Frongillo MF, Franz M, Dominguez Alpizar JL. Discrimination between six species of canine microfilariae by a single polymerase chain reaction. Vet Parasitol. 2006; 135:303–314.

12. Shimizu S, Nojiri K, Matsunaga N, Yamane I, Minami T. Reduction in tick numbers (Haemaphysalis longicornis), mortality and incidence of Theileria sergenti infection in field-grazed calves treated with flumethrin pour-on. Vet Parasitol. 2000; 92:129–138.

13. Shiono H, Yagi Y, Thongnoon P, Kurabayashi N, Chikayama Y, Miyazaki S, Nakamura I. Acquired methemoglobinemia in anemic cattle infected with Theileria sergenti. Vet Parasitol. 2001; 102:45–51.

14. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011; 28:2731–2739.

15. Zhang S, Xu Y, Jin Y, Jin Z, Ju Y, Lu C. Development of a Bereriol slow release agent and its preventive effect against Theileriosis sergenti of cattle. Chin J Vet Sci. 1998; 18:367–369.