Tissue Eng Regen Med.  2018 Oct;15(5):531-546. 10.1007/s13770-018-0152-8.

Click Chemistry-Based Injectable Hydrogels and Bioprinting Inks for Tissue Engineering Applications

• Gopinathan J
• Noh I

• KMID: 2422669
• DOI: http://doi.org/10.1007/s13770-018-0152-8

Abstract

BACKGROUND
The tissue engineering and regenerative medicine approach require biomaterials which are biocompatible, easily reproducible in less time, biodegradable and should be able to generate complex three-dimensional (3D) structures to mimic the native tissue structures. Click chemistry offers the much-needed multifunctional hydrogel materials which are interesting biomaterials for the tissue engineering and bioprinting inks applications owing to their excellent ability to form hydrogels with printability instantly and to retain the live cells in their 3D network without losing the mechanical integrity even under swollen state.
METHODS
In this review, we present the recent developments of in situ hydrogel in the field of click chemistry reported for the tissue engineering and 3D bioinks applications, by mainly covering the diverse types of click chemistry methods such as Diels-Alder reaction, strain-promoted azide-alkyne cycloaddition reactions, thiol-ene reactions, oxime reactions and other interrelated reactions, excluding enzyme-based reactions.
RESULTS
The click chemistry-based hydrogels are formed spontaneously on mixing of reactive compounds and can encapsulate live cells with high viability for a long time. The recent works reported by combining the advantages of click chemistry and 3D bioprinting technology have shown to produce 3D tissue constructs with high resolution using biocompatible hydrogels as bioinks and in situ injectable forms.
CONCLUSION
Interestingly, the emergence of click chemistry reactions in bioink synthesis for 3D bioprinting have shown the massive potential of these reaction methods in creating 3D tissue constructs. However, the limitations and challenges involved in the click chemistry reactions should be analyzed and bettered to be applied to tissue engineering and 3D bioinks. The future scope of these materials is promising, including their applications in in situ 3D bioprinting for tissue or organ regeneration.

Keyword

Click chemistry; Hydrogels; 3D bioprinting; Tissue engineering; Regenerative medicine

MeSH Terms

Biocompatible Materials
Bioprinting*
Click Chemistry
Cycloaddition Reaction
Hydrogel*
Hydrogels*
Ink*
Regeneration
Regenerative Medicine
Tissue Engineering*
Biocompatible Materials
Hydrogel
Hydrogels