Korean J Parasitol.  2018 Jun;56(3):267-274. 10.3347/kjp.2018.56.3.267.

Opisthorchis felineus and Metorchis bilis Metacercariae in Cyprinid Fish Leuciscus idus in Nura-Sarysu River, Kazakhstan

• Kiyan VS
• Bulashev A
• Katokhin A

• KMID: 2416357
• DOI: http://doi.org/10.3347/kjp.2018.56.3.267

Abstract

Aim of the present study was to provide presence of opisthorchiid metacercariae in cyprinid fish Leuciscus idus in Nura-Sarysu river, Kazakhstan. Infection rate of the ides by the metacercariae was 42%. The metacercariae, similar morphologically to those of the liver flukes, were found: elliptical in shape, 0.19-0.25×0.15-0.22 mm, oral and ventral suckers nearly equal size, and excretory bladder O-shape with black content, occupying posterior part of the body. The metacercariae were divided into 2 groups with differences in size and thickness of cyst wall. Adult flukes were recovered from the Syrian hamsters infected with the opisthorch metacercariae and identified with morphological characters to Opisthorchis felineus and Metorchis bilis. DNA sequences of ITS1, ITS2, and cox1 supported the taxonomic assignment.

Keyword

Opisthorchis felineus; Metorchis bilis; Leuciscus idus; metacercariae

MeSH Terms

Adult
Base Sequence
Fasciola hepatica
Humans
Kazakhstan*
Mesocricetus
Metacercariae*
Opisthorchis*
Rivers*
Trematoda
Urinary Bladder