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Lab Anim Res.  2018 Jun;34(2):49-57. 10.5625/lar.2018.34.2.49.

Effects of DA-5513 on alcohol metabolism and alcoholic fatty liver in rats

• Yu JY
• Nguyen HT
• Yong CS
• Park HG
• Jun JH
• Kim JO

• KMID: 2413843
• DOI: http://doi.org/10.5625/lar.2018.34.2.49

Abstract

Hangover is characterized by a number of unpleasant physical and mental symptoms that occur after heavy alcohol drinking. In addition, consistently excessive alcohol intake is considered as a major reason causes liver disease. The present study investigated the in vivo effects of DA-5513 (Morning care® Kang Hwang) on biological parameters relevant to hangover relief and alcoholic fatty liver. Blood alcohol and acetaldehyde concentrations were determined in rats administered a single dose of alcohol and treated with DA-5513 or commercially available hangover relief beverages (Yeomyung® and Ukon®). The effects of DA-5513 on alcoholic fatty liver were also determined in rats fed alcohol-containing Lieber-DeCarli diets for 4 weeks. Serum liver function markers (aspartate and alanine aminotransferase activities) and serum/liver lipid levels were assessed. Blood alcohol and acetaldehyde concentrations were lower in the groups treated with DA-5513 or Yeomyung®, as compared with control rats. However, Ukon® did not produce any significant effects on these parameters. Treatment with DA-5513 significantly reduced serum aspartate and alanine aminotransferase activities and markedly reduced serum cholesterol and triglyceride levels, as compared with control rats. Histological observations using Oil Red O staining found that DA-5513 delayed the development of alcoholic fatty liver by reversing hepatic fat accumulation. These findings suggest that DA-5513 could have a beneficial effect on alcohol-induced hangovers and has the potential to ameliorate alcoholic fatty liver.

Keyword

Morning care®; hangover; acetaldehyde; alcohol-induced fatty liver; hepatic triglyceride

MeSH Terms

Acetaldehyde
Alanine Transaminase
Alcohol Drinking
Alcoholics*
Animals
Aspartic Acid
Beverages
Cholesterol
Diet
Fatty Liver, Alcoholic*
Humans
Liver
Liver Diseases
Metabolism*
Rats*
Triglycerides
Acetaldehyde
Alanine Transaminase
Aspartic Acid
Cholesterol

Figure

• 

  Figure 1 Effects of DA-5513 on serum (A) AST activity and (B) ALT activity in chronic ethanol-treated rats. CON, control diet; ED, alcohol diet; ED+UK, alcohol diet with UK; ED+DA-5513, alcohol diet with DA-5513. The data represent mean±SD (n=10); **P<0.01 and *P<0.05.

• 

  Figure 2 Effects of DA-5513 on serum (A) TG and (B) T-CHO levels in chronic ethanol-treated rats. CON, control diet; ED, alcohol diet; ED+UK, alcohol diet with UK; ED+DA-5513, alcohol diet with DA-5513. The data represent mean±SD (n=10); **P<0.01 and *P<0.05.

• 

  Figure 3 Effects of DA-5513 on hepatic TG in chronic ethanoltreated rats. CON, control diet; ED, alcohol diet; ED+UK; alcohol diet with UK; ED+DA-5513; alcohol diet with DA-5513. The data represent mean±SD (n=10); **P<0.01 and *P<0.05.

• 

  Figure 4 Effects of DA-5513 on hepatic lipid levels in chronic ethanol-treated rats. Liver sections were stained with Oil Red O for histopathological examination. CON, control diet; ED, alcohol diet; ED+UK, alcohol diet with UK; ED+DA-5513, alcohol diet with DA-5513.

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