Korean J Parasitol.
2018 Feb;56(1):11-19. 10.3347/kjp.2018.56.1.11.
Molecular Method Confirms Canine Leishmania Infection Detected by Serological Methods in Non-Endemic Area of Brazil
- Affiliations
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- 1Programa de Pós-Graduação em Patologia, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, Rio Grande do Sul, 90050-170, Brazil. elianedal@ufcspa.edu.br
- 2Programa de Pós-Graduação em Biologia Celular e Molecular, Universidade Luterana do Brasil (ULBRA), Canoas, Rio Grande do Sul, 92425-900, Brazil. mrossett@terra.com.br
- 3Laboratório de Imunologia Celular e Molecular, Programa de Pós-Graduação em Ciências da Saúde, Departamento de Ciências Básicas da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, RS, 90050-170, Brazil.
- 4Centro de Desenvolvimento CientÃfico Tecnológico (CDCT), Fundação Estadual de Produção e Pesquisa em Saúde (FEPPS), Porto Alegre, Rio Grande do Sul, 90610-000, Brazil.
- 5Laboratório Pathos, Porto Alegre, Rio Grande do Sul, 90670-120, Brazil.
- 6Departamento de Microbiologia e Imunologia, Laboratório de Virologia/Diagnóstico Molecular, Instituto de Biociências, Universidade Estadual Paulista (UNESP), Botucatu, São Paulo, 18618-183, Brazil.
- KMID: 2408519
- DOI: http://doi.org/10.3347/kjp.2018.56.1.11
Abstract
- In Brazil, visceral leishmaniasis (VL) is expanding and becoming urbanized, especially in non-endemic areas such as the State of Rio Grande do Sul. Considering that infected dogs are the main reservoir for zoonotic VL, this study evaluated the prevalence of canine visceral leishmaniasis (CVL) in the metropolitan area of Porto Alegre, a new area of expansion of VL in Brazil. Serum and plasma from 405 asymptomatic dogs from the municipalities of Canoas (n=107), São Leopoldo (n=216), and Novo Hamburgo (n=82) were tested for CVL using immunochromatographic (DPP®) and ELISA EIE® assays (2 assays officially adopted by the Brazilian government for the diagnosis of CVL) and real-time PCR to confirm the results. There was no agreement among serological and real-time PCR results, indicating that the Leishmania infection in asymptomatic animals with low parasite load, confirmed by negative parasitological tests (smears and parasite culture), need to be evaluated by molecular methods. The prevalence of LVC in the metropolitan region of Porto Alegre, confirmed by real-time PCR was 4% (5.6% in Canoas and 4.6% in São Leopoldo). The use of molecular method is essential for accurate diagnosis of CVL, especially in asymptomatic dogs in non-endemic areas.
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