Int Neurourol J.  2018 Jan;22(Suppl 1):S23-S33. 10.5213/inj.1834974.487.

Expression of Glial Cell Line-Derived Neurotrophic Factor (GDNF) and the GDNF Family Receptor Alpha Subunit 1 in the Paravaginal Ganglia of Nulliparous and Primiparous Rabbits

• García-Villamar V
• Hernández-Aragón LG
• Chávez-Ríos JR
• Ortega A
• Martínez-Gómez M
• Castelán

• KMID: 2405269
• DOI: http://doi.org/10.5213/inj.1834974.487

Abstract

PURPOSE
To evaluate the expression of glial cell line-derived neurotrophic factor (GDNF) and its receptor, GDNF family receptor alpha subunit 1 (GFRα-1) in the pelvic (middle third) vagina and, particularly, in the paravaginal ganglia of nulliparous and primiparous rabbits.
METHODS
Chinchilla-breed female rabbits were used. Primiparas were killed on postpartum day 3 and nulliparas upon reaching a similar age. The vaginal tracts were processed for histological analyses or frozen for Western blot assays. We measured the ganglionic area, the Abercrombie-corrected number of paravaginal neurons, the cross-sectional area of the neuronal somata, and the number of satellite glial cells (SGCs) per neuron. The relative expression of both GDNF and GFRα-1 were assessed by Western blotting, and the immunostaining was semiquantitated. Unpaired two-tailed Student t -test or Wilcoxon test was used to identify statistically significant differences (P≤0.05) between the groups.
RESULTS
Our findings demonstrated that the ganglionic area, neuronal soma size, Abercrombie-corrected number of neurons, and number of SGCs per neuron were similar in nulliparas and primiparas. The relative expression of both GDNF and GFRα-1 was similar. Immunostaining for both GDNF and GFRα-1 was observed in several vaginal layers, and no differences were detected regarding GDNF and GFRα-1 immunostaining between the 2 groups. In the paravaginal ganglia, the expression of GDNF was increased in neurons, while that of GFRα-1 was augmented in the SGCs of primiparous rabbits.
CONCLUSIONS
The present findings suggest an ongoing regenerative process related to the recovery of neuronal soma size in the paravaginal ganglia, in which GDNF and GFRα-1 could be involved in cross-talk between neurons and SGCs.

Keyword

Pelvic ganglia; Nerve growth factors; Neuronal plasticity; Reproduction; Lower urogenital tract

MeSH Terms

Blotting, Western
Carisoprodol
Female
Ganglia*
Ganglion Cysts
Glial Cell Line-Derived Neurotrophic Factor*
Humans
Nerve Growth Factors
Neuroglia*
Neuronal Plasticity
Neurons
Postpartum Period
Rabbits*
Reproduction
Vagina
Carisoprodol
Glial Cell Line-Derived Neurotrophic Factor
Nerve Growth Factors