Mycobiology.  2017 Dec;45(4):385-391. 10.5941/MYCO.2017.45.4.385.

Antifungical Activity of Autochthonous Bacillus subtilis Isolated from Prosopis juliflora against Phytopathogenic Fungi

• Abdelmoteleb A
• Troncoso-Rojas R
• Gonzalez-Soto T
• González-Mendoza D

• KMID: 2401069
• DOI: http://doi.org/10.5941/MYCO.2017.45.4.385

Abstract

The ability of Bacillus subtilis, strain ALICA to produce three mycolytic enzymes (chitinase, β-1,3-glucanase, and protease), was carried out by the chemical standard methods. Bacillus subtilis ALICA was screened based on their antifungal activity in dual plate assay and cell-free culture filtrate (25%) against five different phytopathogenic fungi Alternaria alternata, Macrophomina sp., Colletotrichum gloeosporioides, Botrytis cinerea, and Sclerotium rolfesii. The B. subtilis ALICA detected positive for chitinase, β-1,3-glucanase and protease enzymes. Fungal growth inhibition by both strain ALICA and its cell-free culture filtrate ranged from 51.36% to 86.3% and 38.43% to 68.6%, respectively. Moreover, hyphal morphological changes like damage, broken, swelling, distortions abnormal morphology were observed. Genes expression of protease, β-1,3-glucanase, and lipopeptides (subtilosin and subtilisin) were confirmed their presence in the supernatant of strain ALICA. Our findings indicated that strain ALICA provided a broad spectrum of antifungal activities against various phytopathogenic fungi and may be a potential effective alternative to chemical fungicides.

Keyword

Bacillus subtilis; Biocontrol; Lipopeptides; Mycolytic enzymes; Prosopis juliflora

MeSH Terms

Alternaria
Bacillus subtilis*
Bacillus*
Botrytis
Chitinase
Colletotrichum
Fungi*
Lipopeptides
Prosopis*
Chitinase
Lipopeptides