Ann Dermatol.  2018 Feb;30(1):119-122. 10.5021/ad.2018.30.1.119.

β-Catenin Regulates the Expression of cAMP Response Element-Binding Protein 1 in Squamous Cell Carcinoma Cells

• Kim SY
• Lee JH
• Sohn KC
• Im M
• Lee Y
• Seo YJ
• Lee JH
• Kim CD

• KMID: 2399775
• DOI: http://doi.org/10.5021/ad.2018.30.1.119

Abstract

No abstract available.

MeSH Terms

Carcinoma, Squamous Cell*
Cyclic AMP Response Element-Binding Protein*
Epithelial Cells*
Cyclic AMP Response Element-Binding Protein

Figure

• 

  Fig. 1 Effects of β-catenin on the growth and maintenance of squamous cell carcinoma (SCC) cells. (A) SCC13 cells were transduced with 10 multiplicity of infections of adenovirus expressing β-catenin for overnight, then fresh growth medium was added. Cells were further incubated for 2 days, then cellular extracts were prepared and analyzed by Western blot. Adenovirus (Ad) expressing LacZ was used as a negative control. (B) SCC13 cells were transduced with a recombinant adenovirus for overnight. After replenish with fresh medium, cells were further incubated in the presence of [3H]thymidine for 2 days. Radioactivity was measured using liquid scintillation counter. The data are represented as percent control. The mean values±standard deviation are averages of triplicate measurements. *p<0.01. (C) SCC13 cells were transduced with a recombinant adenovirus for overnight. Cells were then detached from culture dish and about 2,000 cells were seeded on 100-mm dish and cultured for 2 weeks.

• 

  Fig. 2 cAMP response element binding protein 1 (CREB) as a downstream gene of β-catenin. (A) SCC13 cells were transduced with 10 multiplicity of infections (MOIs) of adenovirus expressing β-catenin for overnight, then fresh growth medium was added. Cells were further incubated for 2 days, then cellular extracts were prepared and analyzed by Western blot. CREB expression was increased in β-catenin overexpressing SCC13 cells. (B) Cells were transduced with a recombinant adenovirus expressing CREB. Overexpression of CREB did not affect the β-catenin expression. (C) SCC13 cells were transduced with a recombinant adenovirus, then cells were detached from culture dish and about 2,000 cells were seeded on 100-mm dish and cultured for 2 weeks. The mean values±standard deviation are averages of three independent experiments. *p<0.01.

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