J Bacteriol Virol.  2017 Sep;47(3):139-147. 10.4167/jbv.2017.47.3.139.

Distributions of Bacillus cereus, Pseudomonas, Enterococcus, and coliforms Isolated from Agricultural Products

• Ham H

• KMID: 2392962
• DOI: http://doi.org/10.4167/jbv.2017.47.3.139

Abstract

A survey was conducted on the bacterial contamination levels from 128 agricultural products, and identified 31 coliforms, 34 Bacillus spp., 35 Pseudomonas spp., and 3 Enterococcus spp.. Antibiotic susceptibility testing of eight Escherichia coli (E. coli) isolates, thirteen Bacillus cereus (B. cereus) isolates and seven Pseudomonas aeruginosa (P. aeruginosa) reveled that none of the E. coli isolates shows multidrug resistance pattern. However, one B. cereus isolate was resistant to Amoxicillin/clavulanic acid + Ampicillin/sulbactam + Cephaloxin, and twelve B. cereus isolates were resistant to Amoxicillin/clavulanic acid + Ampicillin/sulbactam + Cephaloxin + Tetracycline. The data from the current study points out that many agricultural products contain multidrug resistant bacteria, and it can be suggested for the need of proper guidelines and special management on the use of antibiotics in agricultural products.

Keyword

B. cereus; Pseudomonas; Enterococcus; Drug resistance patterns; Agricultural products

MeSH Terms

Anti-Bacterial Agents
Bacillus cereus*
Bacillus*
Bacteria
Drug Resistance, Multiple
Enterococcus*
Escherichia coli
Pseudomonas aeruginosa
Pseudomonas*
Tetracycline
Anti-Bacterial Agents
Tetracycline

Reference

1). Bae YM, Lee SY. Microbial quality of fresh produce in Korea and decontamination technologies. Safe Food. 2009; 4:33–9.

2). Jeon DY, Yoon GB, Yoon YH, Yang SI, Kim JB. Bacterial community of traditional Deonjang in longevity area and antagonistic effect against Bacillus cereus. J Kor Soc Food Sci Nutr. 2016; 45:1035–40.

3). Jung SH, Hur MJ, Ju JH, Kim KA, Oh SS, Go JM, et al. Microbiological evaluation of raw vegetables. J Food Hygi Safety. 2006; 21:250–7.

4). Kim MH, Shin WS. Microbiological quality of raw and cooked foods in middle and high school food service establishments. J Kor Soc Food Sci Nutr. 2008; 37:1343–56.

5). Park WJ, Ryu HY, Lim GY, Lee YD, Park JH. Microbial prevalence and guality of organic farm produce from various production sites. Kor J Food Sci Technol. 2014; 46:262–7.

6). Yoon JS, Moon HW, Lee MA. In vitro activity of antimicrobial combination against multidrug-resistant Peudomonas aeruginosa. Korean J Clin Microbiol. 2006; 9:1–6.

7). Pai HJ. In vitro effects of combined antibiotics against multidrug-resistant Peudomona aeruginosa. Korean J Med. 2010; 79:41–7.

8). Kang CI. Antimicrobial therapy for infections caused by multidrug-resistant gram-nagative bacteria. Korean J Med. 2015; 88:502–8.

9). Choi YJ, Kim HJ. Trends of antimicrobial resistance of Enterococcus faecalis and Enterococcus faecium from clinical specimens between 2005~2007. J Soonchunhyang Med Sci. 2008; 14:183–9.

10). Kim SH, Lim TJ, Kim HY, Park SE, Kim SY. Clinical characteristics of community acquired Enterococcal urinary tract infections in children. J Korean Soc Pediatr Nephrol. 2013; 17:19–24.

11). Lee DY, Kim HE, Lee JS, Kim KS, Cho YS. Comparative evaluation of selective chromogenic media for coliforms bacteria isolated from food. J Food Hygi Safety. 2016; 31:222–5.

12). Ministry of Food and Drug Safety. Korean Food Standards Codex. 2015. 211–30.

13). Ham HJ, Kim SE, Ryu SH, Hwang YO, Choi SM. Bacterial Distributions of Escherichia coli and Bacillus cereus etc. Isolated from Dried Seasoned Marine Products in Garak Fishery Wholesale Market in Seoul, 2009. J Food Hygi Safety. 2010; 25:10–15.

14). Food and Drug Association. Bacteriological analytical manual 7 ^th ed. AOAC International;1992.

15). Marshall RT. Standard methods for the examination of dairy products. Washington D. C.: American Public Health Association. 1993.

16). Choi JW, Park SY, Yeon JH, Lee MJ, Chung DH, Lee KH, et al. Microbial contamination levels of fresh vegetables distributed in markets. J Food Hygi Safety. 2005; 20:43–7.

17). Yu YM, Youn YN, Hua QJ, Cha GH, Lee YH. Biological hazard Analysis of paprikas, Strawberries and tomatoes in the markets. J Food Hygi Safety. 2009; 24:174–81.

18). Kim JS, Bang OK, Chang HC. Examination of microbioogical contamination of ready-to-eat vegetable salad. J Food Hygi Safety. 2004; 19:250–7.

19). Hong CK, Seo YH, Choi CM, Hwang IS, Kim MS. Microbial quality of fresh vegetables and fruits in Seoul, Korea. J Food Hygi Safety. 2012; 27:24–9.

20). Kang TM, Cho SK, Park JY, Song KB, Chung MS, Park JH. Analysis of microbial contamination of sprouts and fresh-cut salads in a market. Korean J Food Sci Technol. 2011; 43:490–4.

21). Seo YH, Jang JH, Moon KD. Microbial evaluation of minimally processed vegetables and sprouts produced in Seoul, Korea. Food Sci Biotechnol. 2010; 19:1283–8.

22). Lim JH, Kim YH, Ahn YT, Kim HU. Studies on the contamination and inhibition of bacillus cereus in domestic raw milk and milk product. Kor J Anim Sci. 2000; 42:215–22.

23). Kim YS, Moon HK. The Microbiological quality of environmentally friendly and ordinary agricultural vegetables used in a school foodservices. J Kor Diet Assoc. 2010; 16:49–61.

24). Lutz JK, Lee JY. Prevalence and antimicrobial-resistance of Pseudomonas aeruginosa in swimming pools and hot tubs. Int J Environ Res Public Health. 2011; 8:554–64.