Korean J Parasitol.  2017 Aug;55(4):451-455. 10.3347/kjp.2017.55.4.451.

Characterization of Echinostoma cinetorchis endoribonuclease, RNase H

Affiliations
  • 1Department of Tropical Medicine and Parasitology, Inha University School of Medicine, Incheon 22212, Korea. tongsookim@inha.ac.kr
  • 2Department of Bioscience, Dongkuk University, Seoul 04620, Korea.
  • 3Department of Infectious Diseases Diagnosis, Incheon Institute of Public Health and Environment, Incheon 22320, Korea.
  • 4Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, School of Public Health, Wonju 26493, Korea.
  • 5Department of Convergence Medicine, University of Ulsan, College of Medicine, Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Korea.
  • 6Department of Biotechnology, Konkuk University, Chungju 27478, Korea.
  • 7Institute of Research and Development, Scorpiogen Co., Anseong 17579, Korea. rainlee67@naver.com

Abstract

Echinostoma cinetorchis is an oriental intestinal fluke causing significant pathological damage to the small intestine. The aim of this study was to determine a full-length cDNA sequence of E. cinetorchis endoribonuclease (RNase H; EcRNH) and to elucidate its molecular biological characters. EcRNH consisted of 308 amino acids and showed low similarity to endoribonucleases of other parasites (<40%). EcRNH had an active site centered on a putative DDEED motif instead of DEDD conserved in other species. A recombinant EcRNH produced as a soluble form in Escherichia coli showed enzymatic activity to cleave the 3"²-O-P bond of RNA in a DNA-RNA duplex, producing 3"²-hydroxyl and 5"²-phosphate. These findings may contribute to develop antisense oligonucleotides which could damage echinostomes and other flukes.

Keyword

Echinostoma cinetorchis; endoribonuclease; recombinant protein; localization; RNase H

MeSH Terms

Amino Acids
Catalytic Domain
DNA, Complementary
Echinostoma*
Endoribonucleases
Escherichia coli
Intestine, Small
Oligonucleotides, Antisense
Parasites
Ribonuclease H*
Ribonucleases*
RNA
Trematoda
Amino Acids
DNA, Complementary
Endoribonucleases
Oligonucleotides, Antisense
RNA
Ribonuclease H
Ribonucleases
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