Korean J Parasitol.  2015 Aug;53(4):477-481. 10.3347/kjp.2015.53.4.477.

Molecular Detection of Giardia intestinalis from Stray Dogs in Animal Shelters of Gyeongsangbuk-do (Province) and Daejeon, Korea

• Shin JC
• Reyes AW
• Kim SH
• Kim S
• Park HJ
• Seo KW
• Song KH

• KMID: 2381766
• DOI: http://doi.org/10.3347/kjp.2015.53.4.477

Abstract

Giardia is a major public health concern and considered as reemerging in industrialized countries. The present study investigated the prevalence of giardiosis in 202 sheltered dogs using PCR. The infection rate was 33.2% (67/202); Gyeongsangbuk-do and Daejeon showed 25.7% (39/152, P<0.0001) and 56% (28/50), respectively. The prevalence of infected female dogs (46.7%, P<0.001) was higher than in male dogs (21.8%). A higher prevalence (43.5%, P<0.0001) was observed in mixed breed dogs than purebred (14.1%). Although most of the fecal samples collected were from dogs of > or =1 year of age which showed only 27.4% positive rate, 61.8% (P<0.001) of the total samples collected from young animals (<1 year of age) were positive for G. intestinalis. A significantly higher prevalence in symptomatic dogs (60.8%, P<0.0001) was observed than in asymptomatic dogs (23.8%). Furthermore, the analysis of nucleotide sequences of the samples revealed that G. intestinalis Assemblages A and C were found in the feces of dogs from Gyeongsangbuk-do and Daejeon. Since G. intestinalis Assemblage A has been known to infect humans, our results suggest that dogs can act as an important reservoir of giardiosis in Korea. Hence, hygienic management should be given to prevent possible transmission to humans.

Keyword

Giardia intestinalis; dog; PCR

MeSH Terms

Animals
Base Sequence
Dog Diseases/epidemiology/*parasitology
Dogs
Feces/parasitology
Female
Genotype
Giardia/classification/genetics/*isolation & purification
Giardiasis/epidemiology/parasitology/*veterinary
Male
Molecular Sequence Data
Protozoan Proteins/genetics
Republic of Korea/epidemiology
Protozoan Proteins