Ann Lab Med.  2017 May;37(3):293-295. 10.3343/alm.2017.37.3.293.

A Puzzling “Switch” in Blood Type Following Blood Transfusion

• Akria L
• Chezar J
• Zisman-Rozen S
• Scheinman
• Zonis Z
• Hoffmann Y
• Falik-Zaccai T
• Kalfon L
• Weiss M
• Braester A
• Suriu C
• Barhoum M
• Kuperman A
• Shaoul E

• KMID: 2369760
• DOI: http://doi.org/10.3343/alm.2017.37.3.293

Abstract

No abstract available.

MeSH Terms

Blood Transfusion*

Figure

• 

  Fig. 1 Forward and reverse ABO/D typing of the patient using a gel technique with the DiaMed IH-1000 Automated Blood Screening System (Bio-Rad). Blood group was determined as: (A) A+ upon arrival, (B) A+ at 12 hr from admission, and (C) O+ at day 7 (post admission). The anti-A column is magnified in all gel cards to emphasize the change from double population (DP) to lack of agglutination.

• 

  Fig. 2 ABO genotyping based on sequence-specific primer (SSP) PCR using RBC Ready Gene ABO kit (Inno-Train DiagnostiK GmbH, Kronberg, Germany). Internal control PCR products are indicated by arrows. (A) Blood from patient upon admission; genotyping consistent with blood type A. (B) Blood from patient 12 hr after arrival; genotyping consistent with blood type A. (C) Blood from patient 7 days post admission; genotyping consistent with blood type O. (D) Patient skin biopsy; genotyping consistent with blood type O. Note: blood type A is distinguished from blood type O based solely on the “non O1” position (*) band.

Reference

1. Oetting WS, Lee HK, Flanders DJ, Wiesner GL, Sellers TA, King RA. Linkage analysis with multiplexed short tandem repeat polymorphisms using infrared fluorescence and M13 tailed primers. Genomics. 1995; 30:450–458. PMID: 8825630.

2. Zivony-Elboum Y, Westbroek W, Kfir N, Savitzki D, Shoval Y, Bloom A, et al. A founder mutation in Vps37A causes autosomal recessive complex hereditary spastic paraparesis. J Med Genet. 2012; 49:462–472. PMID: 22717650.

3. Utter GH, Nathens AB, Lee TH, Reed WF, Owings JT, Nester TA, et al. Leukoreduction of blood transfusions does not diminish transfusion-associated microchimerism in trauma patients. Transfusion. 2006; 46:1863–1869. PMID: 17076839.

4. Utter GH. The microchimerism puzzle. Transfusion. 2012; 52:926–928. PMID: 22571359.